녹차(Green Tea) 캘러스 배양 추출물의 미백 및 피지조절 효능
The Effect of Green Tea Callus Culture Extract for Whitening and Sebum Control
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초록
본 연구에서는 다양한 피부 개선 기능을 가진 녹차를 이용하여 지속가능한 화장품 소재로 개발하기 위하여 캘러스를 유도하고 생산된 녹차 캘러스를 추출하였다. 개발된 녹차캘러스배양추출물은 HPLC-MS/MS 분석을 통해 유효성분(Guanine, Uridine, Adenosine, Guanosine, Tyrosine, Phenylalanine, Tryptophan)이 있음을 확인하였다. 이어 피부 활성에 기여하는 미백 및 피지 조절능의 효능을 평가하여 화장품 소재로써의 가능성을 조사하였다. 그 결과 녹차캘러스배양추출물은 멜라닌 합성에 주요하게 관여하는 것으로 알려진 TYR, MITF 유전자 발현을 저해함으로써 미백 효능에 기여할 것으로 사료되며 피부 유분량의 감소를 통해 피지 개선에 효과가 있을 것으로 판단되었다. 이러한 결과를 바탕으로 신규 기능성 화장품 소재로 활용 가치가 있을 것으로 기대된다.
Abstract
In this study, in order to develop a sustainable cosmetic material using green tea with various skin improvement functions, callus (plant cell) was induced. And the cultured green tea callus was extracted. Plant cell culture technology is a new alternative method for preservation and restoration of plants that can be reproduced continuously and repeatedly without damaging nature. In the developed green tea callus culture extract, nucleic acids such as Guanine, Uridine, Adenosine, Guanosine and amino acids such as Tyrosine, Phenylalanine, and Tryptophan were detected through HPLC-MS2 analysis. These substances are known to be helpful in the physiological activity of skin cells. Subsequently, the efficacy of whitening and sebum control contributing to skin activity was evaluated to investigate the possibility as a cosmetic material. As a result, the green tea callus culture extract was thought to contribute to the whitening effect by inhibiting the expression of TYR and MITF genes known to be mainly involved in melanin synthesis, and it was judged to be effective in improving sebum through the reduction of skin sebum content. Based on these results, it is expected to be useful as a new functional cosmetic material.
참고문헌 (17)
- B. Can, "Globular Embryo Regeneration from Callus in the Tea Plant (Camellia sinensis (L) O. Kuntze)", Indian Journal of Plant Sciences, 11, pp.62~73 (2022)
- B. G. Campling, "Use of the mtt assay for rapid determination of chemosensitivity of human leukemic blast cells", Leukemia Research, 12(10), pp.823~883 (1998)
- 오창종, "녹차와 백차 열수추출물의 피부 주름형성 및 착색 저해 효능", 대한미용학회지, 14(3), pp.257~265 (2018), 대한미용학회[DOI]
- G. H. An, "Current Status and Future Perspectives of Biotechnology Using Plant Tissue Culture Techniques(lll)", J Plant Biotechnol, 20(2), pp.59~62 (1993)
- 한규수, "여정자 추출물의 Tyrosinase 및 MITF 발현 억제 효과", 동의생리병리학회지, 24(2), pp.296~301 (2010), 한의병리학회
- H. N. Christensen, "Role of Amino Acid Transport and Countertransport in Nutrition and Metabolism", Physiol. Rev, 70(1), pp.43~77 (1990)
- J. Arct, "Flavonoids as components of biologically active cosmeceuticals", Clinics in dermatology, 26(4), pp.347~357 (2008)
- 정상욱, "한국인의 피부 수분함유량, 유분 함유량 및 경표피수분손실량의 특성에 관한 연구", 대한화장품학회지, 39(3), pp.233~239 (2013), 사단법인 대한화장품학회
- 이미경, "여대생의 자각하는 유·수분 정도에 따른 안면 피부상태 분석", 아시안뷰티화장품학술지, 11(4), pp.753~759 (2013), 한국피부과학연구원
- M. M. Aboulwafa, "A comprehensive insight on the health benefits and phytoconstituents of Camellia sinensis and recent approaches for its quality control", Antioxidants, 8(10), pp.455~ (2019)
- M. Sutini, "Production of secondary metabolites trimethyl xanthina by Camellia sinensis L suspension culture", AIP Conference Proceedings, 1854(1) (2017)
- S. Khokhar, "Total Phenol, Catechin, and Caffeine Contents of Teas Commonly Consumed in the United Kingdom", J. Agric. Food Chem, 50(3), pp.565~570 (2002)
- Sutini, Widiwurjani, "The production of cinnamic acid secondary metabolites through in vitro culture of callus Camellia sinensis L with the elicitor of cobalt metal ions", AIP Conference Proceedings, 2120(1) (2019)
- T. Efferth, "Biotechnology applications of plant callus cult ures", Engineering, 5(1), pp.50~59 (2019)
- T. Zhao, "Green tea (Camel lia sinensis): A review of its phytochemistry, pharmacolo gy, and toxicology", Molecules, 27(12), pp.3909~ (2022)
- W. Koch, "Applications of tea (Camellia sinensis) and its active con stituents in cosmetics", Molecules, 24(23), pp.4277~ (2019)
- W. Koch, "Black Tea Samples Origin Discrimination Using Analytical Investigation of Secondary Metabolites, Antiradical Scavenging Activity and Chemometric Approach", Molecules, 23(3), pp.513~ (2018)