작약열매추출물의 천연화장품 소재로서 주름 개선 효능에 관한 연구

Abstract

This study aimed to evaluate the potential of Paeoniae Fructus 70% ethanol extract as a natural cosmetic ingredient by examining its antioxidant activity and anti-aging effects using CCD-986sk Cells. The effective concentration of the extract was assessed through cell viability experiments using the 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide(MTT) assay, which revealed over 80% cell viability at concentrations below 25 μg/mL. To assess the ability of the extract to inhibit ROS generation, which contributes to skin aging through UVB absorption, CCD-986sk Cells were exposed to UVB and treated with varying concentrations(6.25, 12.5, 25 μg/mL) of the extract. Results showed a concentration-dependent decrease in ROS levels, with the 25 μg/mL concentration demonstrating antioxidant efficacy similar to the positive control, EGCG.Further investigation explored the connection between MMPs and TIMPs and the impact of elevated pro-collagen levels. a precursor to collagen synthesis, on wrinkle reduction. Evaluation of anti-wrinkle efficacy showed a 29% inhibition of MMP-1 expression, increased TIMP-1 levels with concentration, and a 46% increase in pro-collagen synthesis. Western blot analysis revealed that higher concentrations of the extract inhibited protein expression of MMP-1 and MMP-3 in CCD-986sk Cells, with significant reductions observed across all concentrations(6.25, 12.5, 25 μg/mL). Immunofluorescence staining of collagen indicated increased fluorescence with higher extract concentrations, showing significant results compared to EGCG. Based on these findings, Paeoniae Fructus 70% ethanol extract is suggested to promote the regeneration of UVB-damaged skin cells and protect skin cells, making it a valuable natural ingredient for improving skin aging.