인체피부섬유아세포에서 초석잠 에탄올 추출물의 주름형성 억제 효능
Anti-wrinkle Efficacy of Stachys riederi var. japonica Ethanol Extract in Human Dermal Fibroblasts
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본 연구는 초석잠 뿌리 에탄올 추출물의 주름형성 억제 효능 을 평가하기 위해 항산화 활성, 피부 진피조직 기질단백질 분해 에 관여하는 collagenase와 elastase의 활성 저해효능, 인체피부섬유아세포에서 type I procollagen 합성 촉진능과 MMP-1 유전 자 및 단백질 발현 억제능을 알아보았다. 초석잠 뿌리 에탄올 추출물의 총 폴리페놀 함량은 41.2 mg/ g, 총 플라보노이드 함량은 25.4 mg/g, 전자공여능은 1,000 ppm 에서 82.0%로 나타나 항산화능이 우수하였다. 초석잠 뿌리 에 탄올 추출물은 시험관 내 실험에서 10 mg/mL에서 collagenase 와 elastase 활성을 각각 60.6%, 39.8% 저해하였다. 초석잠 뿌 리 에탄올 추출물의 인체피부섬유아세포에 대한 최대허용농 도는 200 μg/mL 이상으로 독성이 미약하였다. EIA kit를 이용 한 인체피부섬유아세포에서의 type I procollagen 합성능 평가에 서 초석잠 뿌리 에탄올 추출물은 용매대조군에 비해 200 μg/mL 에서 76.6% 유의하게 (p<0.05) 증가시켰다. RT-PCR을 이용한 MMP-1 mRNA 및 단백질 발현에서 초석잠 뿌리 에탄올 추출물 은 대조군에 비해 200 μg/mL에서 각각 20.4%, 9.7% 유의하게 (p<0.05) 감소시켰다. 이상의 결과를 통하여, 초석잠 뿌리 에탄올 추출물은 시험관 내 실험에서 자외선 조사로 유도되는 세포외기질단백분해효소 인 collagenase와 elastase의 활성을 저해시키고, 인체피부섬유 아세포에서 피부 진피조직의 주요 기질단백질인 콜라겐 합성을 증가시키고 제 I형 콜라겐 분해효소인 MMP-1의 유전자 및 단 백질 발현을 감소시킴으로써 광노화로 초래되는 주름형성을 억 제하는 효능이 있음을 확인하였다. 아울러, 이와 같은 주름형성 억제 효능은 초석잠 뿌리 에탄올 추출물의 높은 항산화 활성이 상당 부분 기여한 것으로 생각된다.
Abstract
The objective of this study was to evaluate the anti-wrinkle efficacy of Stachys riederi var. japonica ethanol extract (SREE) in human dermal fibroblasts by analyzing collagen synthesis and collagen degradation. The total polyphenolic and flavonoid contents of SREE were 41.2 and 25.4 mg/g, respectively. At concentrations of 1,000 μg/mL, the electron- donating ability of SREE was 82.0%. In vitro SREE treatment (10 mg/mL) inhibited collagenase and elastase activity by 60.6% and 39.8%, respectively. The maximum permissible level for treating SREE to human dermal fibroblasts was shown to be over 200 μg/mL. In comparison with the vehicle, SREE treatment at 200 μg/mL significantly (p<0.05) increased type I procollagen synthesis by 76.6%. Compared with the control, SREE treatment at 200 μg/mL significantly (p<0.05) decreased matrix metalloproteinase (MMP)-1 mRNA and protein expression by 20.4% and 9.7%, respectively. From these results, we confirmed that SREE had low toxicity and had high anti-wrinkle efficacy in human dermal fibroblasts. Therefore, SREE could be used as a functional cosmetic material for the prevention or alleviation of skin wrinkles induced by ultraviolet rays.
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