미선나무의 저온 추출 공정에 의한 항주름 활성 증진
Enhancement of Anti-wrinkle Activities of Abeliophyllum distichum Nakai through Low Temperature Extraction Process
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Skin anti-wrinkle activities of the stems and leaves of Abeliophyllum distichum Nakai were evaluated by the extracts obtained from various extraction processes such as using hot water at 100 oC, 70% ethanol at 85 oC, and 70% ethanol with ultrasonication at 60 oC The ultrasonicated extract showed 95.62% of the highest cell viability in addition of 0.3㎎/㎖ of the extracts into the normal human fibroblast cell, CCD-986sk. For antioxidant activities, the extracts using ultrasonicated extract showed the highest DPPH free radical scavenging as 80.27%, followed by 75.88% and 62.44% for the extracts using ethanol extract and water extract. The ultrasonicated extract also showed the highest elastase inhibition activity as 25.32%, compared to ethanol extract and water extract based method at 22.01% and 12.88%, respectively. MMP-1 production was most effectively decreased down to 2908.1 pg/㎖ with ultrasonicated extract while 6640.8 pg/㎖ with water extract and 3609.3 pg/㎖ with ethanol extract, in addition of 0.3 ㎎/㎖. Collagen production was increased up to 154.7 ng/㎖ in addition of ultrasonicated extract, and followed by 121.4 ng/㎖ and 31.2 ng/㎖ for ethanol extract and water extract, respectively. These results indicate that the ethanol extract should have skin anti-wrinkling activities and can be improved by the ultrasonication process that high energy input elute more amounts of bioactive substances eluting more amounts of bioactive substances from the high energy input of ultrasonication.
Abstract
Skin anti-wrinkle activities of the stems and leaves of Abeliophyllum distichum Nakai were evaluated by the extracts obtained from various extraction processes such as using hot water at 100 oC, 70% ethanol at 85 oC, and 70% ethanol with ultrasonication at 60 oC The ultrasonicated extract showed 95.62% of the highest cell viability in addition of 0.3㎎/㎖ of the extracts into the normal human fibroblast cell, CCD-986sk. For antioxidant activities, the extracts using ultrasonicated extract showed the highest DPPH free radical scavenging as 80.27%, followed by 75.88% and 62.44% for the extracts using ethanol extract and water extract. The ultrasonicated extract also showed the highest elastase inhibition activity as 25.32%, compared to ethanol extract and water extract based method at 22.01% and 12.88%, respectively. MMP-1 production was most effectively decreased down to 2908.1 pg/㎖ with ultrasonicated extract while 6640.8 pg/㎖ with water extract and 3609.3 pg/㎖ with ethanol extract, in addition of 0.3 ㎎/㎖. Collagen production was increased up to 154.7 ng/㎖ in addition of ultrasonicated extract, and followed by 121.4 ng/㎖ and 31.2 ng/㎖ for ethanol extract and water extract, respectively. These results indicate that the ethanol extract should have skin anti-wrinkling activities and can be improved by the ultrasonication process that high energy input elute more amounts of bioactive substances eluting more amounts of bioactive substances from the high energy input of ultrasonication.
참고문헌 (19)
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