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미소유체 칩 상에서 Quantum Dot 및 마이크로 비드를 이용한 생체물질 분석

Microbead-based bio-assay using quantum dot fluorescence in a microfludic chip

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초록

Abstract

We present a microfluidic chip designed for the detection of antibody by using quantum dots fluorescence and a microbead-based assay. A custom designed PDMS microfluidic chip with multi-layer channel is utilized for capturing microbeads; antibody injection into each micro-well; QD injection; and fluorescence detection. The experiment using the fabricated microfluidic chip has been performed on solutions with various concentrations of antibody and has shown correlated fluorescent intensities.

참고문헌 (12)

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  2. M. S. Kim, "Fabrication and characteristics of MOSFET protein sensor using gold-black gate", 14(3), pp.137~143 (2005)
  3. H.-C. Yeh, "Single bio-molecule detection with quantum dots in a microchannel", pp. 371-374, pp.2004~ (2004)
  4. E. R. Goldman, "Multiplexed toxin analysis using four colors of quantum dot fluororeagents", pp.684~688 (2004)
  5. K.-S. Yun, "High-throughput bio-molecule detection using microbead-based assay with quantum dot fluorescence in a microfluidic chip Proceedings of International Conference on Miniaturized Systems for Chemistry and Life Sciences", pp.222~224 (2004)
  6. X. Gao, "Quantum dot-encoded mesoporous beads with high brightness and uniformity", 76, pp.2406~2410 (2004)
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  8. J.-W. Choi, "and C. H. Ahn An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities", 2(1), pp.27~30 (2002)
  9. K. Sato, "Determination of carcinoembryonic antigen in human sera by integrated bead-bed immunoasay in a microchip for cancer diagnosis", 73(6), pp.1213~1218 (2001)
  10. J. H. Thomas, "Bead-based electrochemical immunoassay for bacteriophage MS2", 76(10), pp.2700~2707 (2004)
  11. T. Buranda, "Biomolecular recognition on well-characterized beads packed in microfluidic channels", 74(5), pp.1149~1156 (2002)
  12. M. F. Ali, "DNA hybridization and discrimination of single-nucleotide mismatches using chip-based microbead arrays", 75(18), pp.4732~4739 (2003)

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