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Microfluidic immunoassay using superparamagnetic nanoparticles inan enhanced magnetic field gradient
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Abstract
This paper reports a novel immunoassay method using superparamagnetic nanoparticles and an enhanced magnetic field gradient for the detection of protein in a microfluidic device. We use superparamagnetic nanoparticles as a label and fluorescent polystyrene beads as a solid support. Based on this platform, magnetic force-based microfluidic immunoassay is successfully applied to analyze the concentration of IgG as model analytes. In addition, we present ferromagnetic microstructure connected with a permanent magnet to increase magnetic flux density gradient (dB/dx, ~104 T/m), which makes limit of detection reduced. The detection limit is reduced to about 1pg/mL.
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