실시간 박테리아 감지를 위한 정전용량방식의 MEMS 바이오센서
MEMS based capacitive biosensor for real time detection of bacterial growth
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A biosensor based on the measurement of capacitance changes has been designed and fabricated for simple and real-time detection of bacteria. Compared to an impedance measurement technique, the capacitance measurement can makeadditional measurement circuits simpler, which improves a compatability for integration between the sensor and circuit.The fabricated sensor was characterized by detecting Escherichia coli(E. coli). The capacitance changes measured by thesensor were proportional to E. coli cell density, and the proposed sensor could detect 1×106 cfu/ml E. coli at least. Thereal-time detection was verified by measuring the capacitance every 20 minutes. After 7 hours of E. coli growthexperiment, the capacitance of the sensor in the micro volume well with 4.5×105 cfu/ml of initial E. coli density increasedby 20 pF, and that in another wells with 1.5×106 cfu/ml and 8.5×107 cfu/ml initial E. coli density increased by 56 pFand 71 pF, respectively. The proposed sensor has a possibility of the real-time detection for bacterial growth, and candetect E. coli cels with 1.8×105 cfu in nutrient broth in 5 hours.
Abstract
A biosensor based on the measurement of capacitance changes has been designed and fabricated for simple and real-time detection of bacteria. Compared to an impedance measurement technique, the capacitance measurement can makeadditional measurement circuits simpler, which improves a compatability for integration between the sensor and circuit.The fabricated sensor was characterized by detecting Escherichia coli(E. coli). The capacitance changes measured by thesensor were proportional to E. coli cell density, and the proposed sensor could detect 1×106 cfu/ml E. coli at least. Thereal-time detection was verified by measuring the capacitance every 20 minutes. After 7 hours of E. coli growthexperiment, the capacitance of the sensor in the micro volume well with 4.5×105 cfu/ml of initial E. coli density increasedby 20 pF, and that in another wells with 1.5×106 cfu/ml and 8.5×107 cfu/ml initial E. coli density increased by 56 pFand 71 pF, respectively. The proposed sensor has a possibility of the real-time detection for bacterial growth, and candetect E. coli cels with 1.8×105 cfu in nutrient broth in 5 hours.
참고문헌 (16)
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