임피던스 측정을 이용한 세포의 변형성 분석용 미소유체 칩
Microfluidic chip for characterization of mechanical property of cell by using impedance measurement
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초록
In this paper we propose a microfluidic chip that measures the mechanical stiffness of cell membrane using impedance measurement. The microfluidic chip is composed of PDMS channel and a glass substrate with electrode. The proposed device uses patch-clamp technique to capture and deform a target cell and measures impedance of deformed cells. We demonstrated that the impedance increased after the membrane stretched and blocked the channel.
Abstract
In this paper we propose a microfluidic chip that measures the mechanical stiffness of cell membrane using impedance measurement. The microfluidic chip is composed of PDMS channel and a glass substrate with electrode. The proposed device uses patch-clamp technique to capture and deform a target cell and measures impedance of deformed cells. We demonstrated that the impedance increased after the membrane stretched and blocked the channel.
참고문헌 (19)
- L.-S. Jang, "Microfluidic device for cell capture and impedance measurement", Biomed. Microdevice, 9, pp.737~743 (2007)
- S. Zheung, "Micro coulter counters with platinum black electroplated electrodes for human blood cell sensing", Biomed. Microdevices, 10, pp.221~231 (2008)
- L.-M. Fu, "Fabrication and testing of high-performance detection sensor for capillary electrophoresis microchip", Biomed. Microdevices, 10, pp.73~80 (2008)
- S. Gawad, "Dielectric spectroscopy in a micromachined flow cytometer : Theoretical and practical considerations", Lab Chip, 4, pp.241~251 (2004)
- T. Sun, "Broadband single cell impedance spectroscopy using maximum length sequences : theoretical analysis and practical considerations", Meas. Sci. Technol, 18, pp.2859~2868 (2007)
- R. Pantoja, "Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics", Biosens. Bioelectron., 20(3), pp.509~517 (2004)
- N. Fertig, "Activity of single ion channel proteins detected with a planar microstructure", Appl. Phys. Lett., 9(25), pp.4865~4867 (2002)
- T. Lehnert, "Realization of hollow SiO2 micronozzles for electrical measurements of living cells", Appl. Phys. Lett., 81(26), pp.5063~5065 (2002)
- J. Seo, "Integrated multiple patch-clamp array chip via lateral cell trapping junctions", Appl. Phys. Lett., 84(11), pp.1973~1975 (2004)
- A. Y. Lau, "Open-access microfluidic patch-clamp array with raised lateral cell trapping sites", Lab Chip, 6, pp.1510~1515 (2006)
- C. Chen, "A high-performance elastomeric patch clamp chip", Lab Chip, 6, pp.1338~1345 (2006)
- E. Evans, "Apparent viscosity and cortical tension of blood granulocytes determined by micropipet aspiration", Biophys. J., 56, pp.151~160 (1989)
- M. Dao, "Mechanics of the human red blood cell deformed by optical tweezers", J. Mech. Phys. Solids., 51, pp.2259~2280 (2003)
- J. P. Mills, "Nonlinear elastic and viscoelastic deformation of the red blood cell induced by optical tweezers", Mech. Chem. Biosyst., 1, pp.169~180 (2004)
- . Suresh, "Biomechanics and biophysics of cancer cells", Acta Biomater., 3, pp.413~438 (2007)
- 정귀상, "PDMS 몰드를 이용한 초고온 MEMS용 SiCN 미세구조물 제작과 그 특성", 센서학회지, 15(1), pp.53~57 (2006), 한국센서학회
- 윤광석, "미소유체 칩 상에서 Quantum Dot 및 마이크로 비드를 이용한 생체물질 분석", 센서학회지, 14(5), pp.308~312 (2005), 한국센서학회
- J. Guck, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence", Biophys. J., 88, pp.3689~3698 (2005)
- H. Morgen, "Single cell dielectric spectroscopy", J. Phys. D: Appl. Phys., 40, pp.61~70 (2007)