일회용 스트립형 효소면역센서용 플랫폼의 개발
Development of Disposable Enzyme-linked Immunosensor Strip Platform
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study introduced the development of a strip type disposable enzyme-linked immunosensor platform for the detection of IgG. Strips of the strip sensor were fabricated by using commercial nitrocellulose filter membranes and a housing holder for the strips was manufactured by using a standard injection molding process for a plastic material. An IgG-urease conjugate was prepared and used for the competitive immune-binding with sample IgG. From the enzymatic reaction between the conjugated urease and urea added, ammonia was generated and caused a localized alkaline pH change on the immobilized antibody band which was coated onto the sensor strips. This pH increase subsequently caused a color change of the antibody band in the presence of a pH indicator, phenol red. Used in conjunction with a competitive immunoassay format, the intensity of the color produced is directly linked with the concentration of target analyte, IgG, and specific measurement of IgG in a lateral flow immunoassay format was achieved over the range 100 ppb to 2000 ppb IgG.
Abstract
This study introduced the development of a strip type disposable enzyme-linked immunosensor platform for the detection of IgG. Strips of the strip sensor were fabricated by using commercial nitrocellulose filter membranes and a housing holder for the strips was manufactured by using a standard injection molding process for a plastic material. An IgG-urease conjugate was prepared and used for the competitive immune-binding with sample IgG. From the enzymatic reaction between the conjugated urease and urea added, ammonia was generated and caused a localized alkaline pH change on the immobilized antibody band which was coated onto the sensor strips. This pH increase subsequently caused a color change of the antibody band in the presence of a pH indicator, phenol red. Used in conjunction with a competitive immunoassay format, the intensity of the color produced is directly linked with the concentration of target analyte, IgG, and specific measurement of IgG in a lateral flow immunoassay format was achieved over the range 100 ppb to 2000 ppb IgG.
참고문헌 (16)
- K. Ryan, "Sherris Medical Microbiology", pp.137~142 (2004), McGraw Hill
- T. Parish, "Mycobacterium: Genomics and Molecular Biology", pp.231~240 (2009), Caister Academic Press
- K. Janssen, "Implementation of real-time RTPCR for detection of human metapneumovirus and its comparison with enzyme immunoassay", Archiv. Virology, 155, pp.207~215 (2010)
- E. Brindle, "Serum,plasma,and dried blood spot high-sensitivityC-reactive protein enzyme immunoassay forpopulation research", J. Immunological Methods, 362, pp.112~120 (2010)
- M. Diaz-Gonzalez, "Immunosensor for mycobacterium tuberculosis on screen-printed carbon electrodes", Biosens. & Bioelectron, 20, pp.2035~2043 (2005)
- 장승철, "Disposable in-field electrochemical potable sensor system for free available chlorine (FAC) detection", 센서학회지, 16(6), pp.449~456 (2007), 한국센서학회
- 장승철, "제초제 검출을 위한 전기화학적 일회용 면역센서", 센서학회지, 20(1), pp.35~39 (2011), 한국센서학회
- C. Medina-Torres, "Validation of a commercial enzyme immunoassayfor detection of clostridium difficile toxins in feces ofhorses with acute diarrhea", J. Veteri. InternalMedicine, 24(3), pp.628~632 (2010)
- Seong Jung Kwon, "Application of Polyaniline to an Enzyme-Amplified Electrochemical Immunosensor as an Electroactive Report Molecule", Bulletin of the Korean Chemical Society, 31(11), pp.3103~3108 (2010), 대한화학회
- M.Oellerich, "Enzyme-immunoassay-a review", J Clinic. Chem. & Clinic. Biochem, 22(12), pp.895~904 (1984)
- R. Okennedy, "A review of enzyme-immunoassay and adescription of a competitive enzyme-linkedimmunosorbent-assay for the detection ofimmunoglobulin concentrations", Biochem. Edu, 18(3), pp.136~140 (1990)
- E. Garet, "Comparative evaluation of enzyme-linked immunoassay and reference methods for the detection of shellfish hydrophilic toxins in several presentations of seafood", J. Agricul. Food Chem, 58(3), pp.1410~1415 (2010)
- W. Koh, "Monitoring the activation of neuronal nitric oxidesynthase in brain tissue and cells with a potentiometricimmunosensor", Biosens. Bioelectron, 25, pp.211~217 (2009)
- Young-Kee Kim, "Immuno-strip biosensor system to detect enrofloxacin residues", Journal of Industrial and Engineering Chemistry, 15(2), pp.229~232 (2009), 한국공업화학회
- "Enzyme-linked immuno-strip biosensor to detect Escherichia coli O157 : H7", ULTRAMICROSCOPY, 108(10), pp.1348~1351
- Seung Jae Yi, "An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG", Bulletin of the Korean Chemical Society, 32(4), pp.1298~1302 (2011), 대한화학회