폐암 바이오마커 검출용 나노SPR 바이오센서
Nano SPR Biosensor for Detecting Lung Cancer-Specific Biomarker
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초록
In this research, we developed a biosensor to detect lung cancer-specific biomarker using Anodic Aluminum Oxide (AAO) chip based on interference and nano surface plasmon resonance (nanoSPR). The nano-porous AAO chip was fabricated 2 μm of pore-depth by twostep anodizing method for surface uniformity. NanoSPR has sensitivity to the refractive index (RI) of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized to the Au-deposited surface of nano-porous AAO chip. To detect the lung cancer-specific biomarker, antibodies were immobilized on the surface of the chip by Self Assembled Monolayer (SAM) method. Since then lung cancer-specific biomarker was applied atop the antibodies immobilized layer. The specific reaction of the antigen-antibody contributed to the change in the refractive index that cause shift of resonance spectrum in the interference pattern. The Limit of Detection (LOD) was 1 fg/ml by using our nano-porous AAO biosensor chip.
Abstract
In this research, we developed a biosensor to detect lung cancer-specific biomarker using Anodic Aluminum Oxide (AAO) chip based on interference and nano surface plasmon resonance (nanoSPR). The nano-porous AAO chip was fabricated 2 μm of pore-depth by twostep anodizing method for surface uniformity. NanoSPR has sensitivity to the refractive index (RI) of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized to the Au-deposited surface of nano-porous AAO chip. To detect the lung cancer-specific biomarker, antibodies were immobilized on the surface of the chip by Self Assembled Monolayer (SAM) method. Since then lung cancer-specific biomarker was applied atop the antibodies immobilized layer. The specific reaction of the antigen-antibody contributed to the change in the refractive index that cause shift of resonance spectrum in the interference pattern. The Limit of Detection (LOD) was 1 fg/ml by using our nano-porous AAO biosensor chip.
참고문헌 (18)
- Kumar V, "Robbins Basic Pathology" (2003), Saunders
- Boring, C. C., "Cancer statistics, 1993", CA Cancer J. Clin, 43, pp.7~26 (1993)
- Berlin, N. I., "The national cancer institute cooperative early lung cancer detection program", Am. Rev. Respir. Dis, 130, pp.545~549 (1984)
- Tockman, M. S., "Survival and mortality from lung cancer in a screened population", Chest, 89, pp.324S~325S (1986)
- WCS Cho, "Serum amyloid A is elevated in the serum of lung cancer patients with poor prognosis", British Journal of Cancer, 102(12), pp.1731~1735 (2010)
- Emiliano Cocco, "Serum amyloid A : A novel biomarker for endometrial cancer", Cancer, 116(4), pp.843~851 (2010)
- Howard BA, "Identification and validation of a potential lung cancer serum biomarker detected by matrix-assisted laser desorption", ionization-time of flight spectra analysis, 2003, pp.3, 1720~1724
- Hutter, E, "Exploitation of localized surface plasmon resonance", Adv. Mater, 16, pp.1685~1706 (2004)
- L. C. Archibald, "The anodic oxidation of zirconium I. growth stresses in anodic ZrO2 films", Electrochimica Acta, 22, pp.15~20 (1977)
- J. H. Kim, "The design and fabrication of the accelerat ion sensor using the cantilever and the Fabry-Perot interferometer", 석사, pp.2002
- S. H. Yeom, "Development of LSPR based biosensor using Au deposited nano-porous AAO chip", Sens. Lett, 9(1), pp.92~96 (2011)
- J. D. Edward, "The structure of Anodic oxide coatings", Trans. Am. Inst. Min. Metall. Eng, 156, pp.288~ (1944)
- H. Masuda, "Ordered metal nanohole arrays made by a two-step replication of honeycomb structures of anodic alumina", Science, 268(5216), pp.1466~1468 (1955)
- S. H. Yeom, "Highly sensitive nano-porous lattice biosensor based on localized surface plasmon resonance and interference", Optics Express, 19(23), pp.22882~22891 (2011)
- H. Liu, "Enhanced surface plasmon resonance on a smooth silver film with a seed growth layer", ACS Nano, 4(6), pp.3139~3146 (2010)
- Ahmed Busnaina, "Nanomanufacturing Handbook", pp.33~50 (2006), CRC Press
- Y. S. Chi, "Surface engineering based on self-assembled monolayers", Polymer Science and Technology, 17, pp.172~181 (2006)
- D. S. Kim, "Regression Analysis", pp.18~31 (1994), Bobmunsa