반사광 측정 모드에서 금과 은을 사용한 이층 금속 칩과 삼층 금속 칩의 특성 연구
Characteristics of Gold and Silver Based Bi- and Tri-metallic SPR Chip in the Intensity Measurement Mode
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Characteristics of the conventional gold (Au) surface plasmon resonance (SPR) chip, bi-metallic(Au/silver (Ag)) SPR chip, and tri-metallic(Au/Ag/Au) SPR chip were investigated and compared in the intensity measurement mode for the enhancement of SPR image sensor reactivity. Reflectance curves of the Au, bi- and tri-metallic SPR chips were acquired in phosphate-buffered saline (PBS) solution and were compared. The line width of the reflectance curve of the bi-metallic chip was the narrowest among the three different types of the chips. Also, the tangential slope of the bi-metallic chip was steeper than those of the other chips. Various concentrations of bovine serum albumin (BSA) were utilized in the SPR experiment. As a result, among the above three chips reflectance variation value of the bi-metallic chip was the largest.
Abstract
Characteristics of the conventional gold (Au) surface plasmon resonance (SPR) chip, bi-metallic(Au/silver (Ag)) SPR chip, and tri-metallic(Au/Ag/Au) SPR chip were investigated and compared in the intensity measurement mode for the enhancement of SPR image sensor reactivity. Reflectance curves of the Au, bi- and tri-metallic SPR chips were acquired in phosphate-buffered saline (PBS) solution and were compared. The line width of the reflectance curve of the bi-metallic chip was the narrowest among the three different types of the chips. Also, the tangential slope of the bi-metallic chip was steeper than those of the other chips. Various concentrations of bovine serum albumin (BSA) were utilized in the SPR experiment. As a result, among the above three chips reflectance variation value of the bi-metallic chip was the largest.
참고문헌 (13)
- Analysis for new drugs R&D activation, "E. J. Kim", Biotech Policy Research Center, ~ (2010)
- The pharmaceutical industry R & D, Present and Future, "K. H. Lee", Korea Pharmaceuticals Association, ~ (2015)
- Alvydas Mikulskis, "Solution ELISA as a platform of choice for development of robust, drug tolerant immunogenicity assays in support of drug development", Journal of Immunological Methods, 365, pp.38~49 (2011)
- Mei-Feng Lee, "Study on the LAMP-PCR-hybridization-thermal melt-ELISA method for molecular detection of multidrug resistance in Mycobacterium tuberculosis isolates", Biomarkers and Genomic Medicine, 6, pp.126~132 (2014)
- Yulin Chen, "On-chip synthesis of RNA aptamer microarrays for multiplexed protein biosensing with SPR imaging measurements", Langmuir, 28, pp.8281~8285 (2012)
- Jennifer B. Fasoli, "Surface enzyme chemistries for ultrasensitive microarray biosensing with SPR imaging", Langmuir, 31, pp.9527~9536 (2015)
- Zhiyou Wang, "Stable and sensitive silver surface plasmon resonance imaging sensor using trilayered metallic structures", Analytical Chemistry, 86, pp.1430~1436 (2014)
- 김형진, "반사광 측정 모드에서 금과 은의 쌍금속 표면 플라즈몬 공명 칩의 특성과 임계각을 이용한 굴절률 계산", 센서학회지, 24(6), pp.423~428 (2015), 한국센서학회[DOI]
- Y. K. Lee, "Detection of amyloid-β42 using a waveguide coupled bimetallic surface plasmon resonance sensor chip in the intensity measurement mode", PLoS ONE, 9, pp.e98992~ (2014)
- E. Kretschmann, "Radiative decay of non-radiative surface plasmons excited by light", Zeitschrift für Naturforschung A, 23, pp.2135~2136 (1968)
- Gopa Mandal, "Interaction of bovine serum albumin and albumin-gold nanoconjugates with L-aspartic acid. A spectroscopic approach", Colloids and Surfaces B : Biointerfaces, 81, pp.178~184 (2010)
- 배순언, "C18-PEG로 화학흡착된 실리콘 웨이퍼의 표면특성 및 선택적 알부민 흡착", 생체재료학회지, 12(4), pp.167~173 (2008), 한국생체재료학회