The Fluorescence Immunoassay of lung Cancer Serum Diomarkers using Quantum dots
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Cancer serum biomarkers have advanced our ability to more accurately predict tumor classification, prognostic/metastatic potential, and response potential to novel chemotherapies. Serum amyloid A (SAA) and Vascular endothelial growth factor (VEGF) have potential utility as a serum biomarker for lung cancer. Quantum dots, nanometer-sized crystals, have a high quantum yield, sensitivity, and pronounced photostability. The properties of quantum dots can be efficiently applied to the detection of serum biomarkers in immunoassays as fluorescent probe. We used quantum dots as fluorescent probes in immunoassays and attempted to detect serum amyloid A and vascular endothelial growth factor as serum biomarkers of lung cancer. This fluorescence immunoassay based on the properties of quantum dots is applicable to the detection of serum biomarkers for lung cancer. The fluorescence immunoassay with quantum dots should allow the efficient and specific detection of serum amyloid A (SAA) for the possible diagnosis of lung cancer
Abstract
Cancer serum biomarkers have advanced our ability to more accurately predict tumor classification, prognostic/metastatic potential, and response potential to novel chemotherapies. Serum amyloid A (SAA) and Vascular endothelial growth factor (VEGF) have potential utility as a serum biomarker for lung cancer. Quantum dots, nanometer-sized crystals, have a high quantum yield, sensitivity, and pronounced photostability. The properties of quantum dots can be efficiently applied to the detection of serum biomarkers in immunoassays as fluorescent probe. We used quantum dots as fluorescent probes in immunoassays and attempted to detect serum amyloid A and vascular endothelial growth factor as serum biomarkers of lung cancer. This fluorescence immunoassay based on the properties of quantum dots is applicable to the detection of serum biomarkers for lung cancer. The fluorescence immunoassay with quantum dots should allow the efficient and specific detection of serum amyloid A (SAA) for the possible diagnosis of lung cancer
참고문헌 (27)
- B.K. Edwards, "Annual report to the nation on the status of cancer, 1973-1999, featuring implications of age and aging on U.S. cancer burden", Cancer, 15(94), pp.2766~2792 (2002)
- A. Jemal, "Cancer statistics 2003", CA Cancer J. Clin, 53(1), pp.5~26 (2003)
- T.D. Chanin, "Recent developments in biomarkers for the early detection of lung cancer: perspectives based on publications 2003 to present", Curr Opin Pulm Med, 10(4), pp.242~247 (2004)
- W.M. Gao, "Distinctive serum protein profiles involving abundant proteins in lung cancer patients based upon antibody microarray analysis", BMC Cancer, 23(5), pp.110~ (2005)
- N. Upragarin, "Extrahepatic production of acute phase serum amyloid A", Histol Histopathol, 20(4), pp.1295~1307 (2005)
- B.A. Howard, "Identification and validation of a potential lung cancer serum biomarker detected by matrix-assisted laser desorption", ionization-time of flight spectra analysis, 2003, pp.3(9), 1720~1724
- W.C. Cho, "Identification of serum amyloid a protein as a potentially useful biomarker to monitor relapse of nasophary- ngeal cancer by serum proteomic profiling", Clin. Cancer Res, 1(10), pp.43~52 (2004)
- N, Khan, "Clinical utility of serum amyloid A and macrophage migration inhibitory factor as serum biomarkers for the detection of nonsmall cell lung carcinoma", Cancer, 101(2), pp.379~384 (2004)
- E. Laack, "Pretreatment vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) serum levels in patients with metastatic non-small cell lung cancer (NSCLC)", Lung Cancer, 50(1), pp.51~58 (2005)
- W. Lian, "Ultrasensitive detection of biomolecules with fluorescent dye-doped nanoparticles", Anal. Biochem, 334(1), pp.135~144 (2004)
- A.M. Smith, "Chemical analysis and cellular imaging with quantum dots", Analyst, 129(8), pp.672~677 (2004)
- M.P. Bruchez, "Turning all the lights on: quantum dots in cellular assays", Curr Opin Chem Biol, 9(5), pp.533~537 (2005)
- X. Michalet, "Quantum dots for live cells, in vivo imaging, and diagnostics", Science, 307(5709), pp.538~544 (2005)
- M. Jr. Bruchez, "Semiconductor nanocrystals as fluorescent biological labels", Science, 281(5385), pp.2013~2016 (1998)
- W.C. Chan, "Quantum dot bioconjugates for ultrasen- sitive nonisotopic detection", Science, 281(5385), pp.2016~2018 (1998)
- J.K. Jaiswal, "Potentials and pitfalls of fluore- scent quantum dots for biological imaging", Trends Cell Biol, 14(9), pp.497~504 (2004)
- C.Z. Hotz, "Applications of quantum dots in biology: an overview", Methods Mol. Biol, 303, pp.1~17 (2005)
- T. Liu, "The fluorescence bioassay platforms on quantum dots nanoparticles", J. Fluoresc, 15(5), pp.729~733 (2005)
- E.R. Goldman, "Multiplexed toxin analysis using four colors of quantum dot fluororeagents", Anal. Chem, 76(3), pp.684~688 (2004)
- E.R. Goldman, "Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays", Anal. Chem, 74(4), pp.841~847 (2002)
- E.R. Goldman, "Avidin: A Natural Bridge for Quantum Dot-Antibody Conjugates", J. Am. Chem. Soc, 124(22), pp.6378~6382 (2002)
- B.M. Lingerfelt, "Preparation of Preparation of quantum dot-biotin conjugates and their use in immunochromatography assays", Anal. Chem, 75(16), pp.4043~4049 (2003)
- U. Manne, "Recent advances in biomarkers for cancer diagnosis and treatment", Drug Discov Today, 10(14), pp.965~976 (2005)
- I.L. Medintz, "Quantum dot bioconjugates for imaging, labelling and sensing", Nat. Mater, 4(6), pp.435~446 (2005)
- F. Pinaud, "Advances in fluorescence imaging with quantum dot bio-probes", Biomaterials, 27(9), pp.1679~1687 (2006)
- D. Geho, "Pegylated, steptavidin- conjugated quantum dots are effective detection elements for reverse-phase protein microarrays", Bioconjug Chem, 16(3), pp.559~566 (2005)
- D.H. Liu, "Serum amyloid A protein: a potential biomarker correlated with clinical stage of lung cancer", Biomed Environ Sci, 20(1), pp.33~40 (2007)