닌히드린 용액의 저온 건조에 의한 프롤린 검출을 위한종이기반 센서의 분해능 개선
Improved Resolution of Paper-based Sensor for Proline Detection by Low-temperature Drying of Ninhydrin Solution
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, we describe the improvement of the resolution of a paper-based sensor by fabricating a high-concentration ninhydrin part using a low-temperature drying method to detect proline with high resolution. In the conventional paper-based sensor for detecting proline, the ninhydrin part is fabricated at room temperature, and in this process, the ninhydrin solution spreads around the ninhydrin part. Therefore, the concentration of the ninhydrin part becomes lower than that of the applied solution, lowering the resolution of the sensor. The proposed paper-based sensor better improved the sensitivity of the sensor compared to the existing sensor by fabricating a high-concentration ninhydrin part through drying the ninhydrin solution using a low-temperature drying method. Owing to the experiment, the intensity of the green color of the paper-based sensor with the integrated ninhydrin part fabricated at 10℃ is approximately 20% lower than the paper-based sensor with an integrated ninhydrin part fabricated at room temperature, indicating better sensor resolution. Therefore, the paper-based sensor with an integrated ninhydrin part fabricated at a high concentration could be useful for diagnosing drought.
Abstract
In this study, we describe the improvement of the resolution of a paper-based sensor by fabricating a high-concentration ninhydrin part using a low-temperature drying method to detect proline with high resolution. In the conventional paper-based sensor for detecting proline, the ninhydrin part is fabricated at room temperature, and in this process, the ninhydrin solution spreads around the ninhydrin part. Therefore, the concentration of the ninhydrin part becomes lower than that of the applied solution, lowering the resolution of the sensor. The proposed paper-based sensor better improved the sensitivity of the sensor compared to the existing sensor by fabricating a high-concentration ninhydrin part through drying the ninhydrin solution using a low-temperature drying method. Owing to the experiment, the intensity of the green color of the paper-based sensor with the integrated ninhydrin part fabricated at 10℃ is approximately 20% lower than the paper-based sensor with an integrated ninhydrin part fabricated at room temperature, indicating better sensor resolution. Therefore, the paper-based sensor with an integrated ninhydrin part fabricated at a high concentration could be useful for diagnosing drought.
참고문헌 (15)
- L. Szabados, "Proline : a multifunctional amino acid", Trends plant Sci, 15(2), pp.89~97 (2010)
- P. B. K. Kishor, "Role of proline in cell wall synthesis and plant development and its implications in plant ontogecy", Front Plant Sci, 6, pp.00544~ (2015)
- S. Hayat, "Role of proline under changing environments environments", Plant Signal Behav, 7(11), pp.1456~1466 (2012)
- P. B. K. Kishor, "Regulation of proline biosynthesis, degradation, uptake and transport in higher plants : Its implications in plant growth and abiotic stress tolerance", Curr. Sci, 88(3), pp.424~438 (2005)
- H. M. Akram, "Impact of water deficit stress on various physiological and agronomic traits of three basmati rice(Oryza sativa L. )Cultivars", J. Anim. Plant. Sci, 23(5), pp.1415~1423 (2013)
- P. Thakur, "Cold stress effects on reproductive development in grain crops : An overview", Environ. Exp. Bot, 67(3), pp.429~443 (2010)
- S. I. Zandalinas, "Plant adaptations to the combination of drought and high temperatures", Physiol. Plantarum, 162(1), pp.2~12 (2018)
- R. Mittler, "Genetic engineering for modern agriculture : challenges and perspectives", Annu. Rev. Plant. Biol, 61(1), pp.443~462 (2010)
- J. S. Boyer, "Plant productivity and environment", Science, 218(4571), pp.443~448 (1982)
- E. Abrahám, "Methods for determination of proline in plants", Methods Mol. Biol, 639, pp.317~331 (2010)
- G. Noctor, "Simultaneous measurement of foliar glutathione, gamma-glutamylcysteine, and amino acids by high-performance liquid chromatography : comparison with two other assay methods for glutathione", Anal. Biochem, 264(1), pp.98~110 (1998)
- I. Smith, "Colour reactions on paper chromatograms by a dipping technique", Nat, 171, pp.43~44 (1953)
- F. P. Chinard, "Photometric estimation of proline and ornithine", J. Biol. Chem, 199(1), pp.91~95 (1952)
- Y. S. Choi, "Detection of proline using a novel paper-based analytical device for on-site diagnosis of drought stress in plants", Rev. Sci. Instrum, 90(4), pp.045002~ (2019)
- Y. S. Choi, "Highly sensitive enclosed multilayer paper-based microfluidic sensor for quantifying proline in plants", Analytica Chimica Acta, 1105, pp.169~177 (2020)