휘발성 질소화합물 감응형 색변환 센서를 활용한 연어 신선도 모니터링
Freshness Monitoring of Raw Salmon Filet Using a Colorimetric Sensor that is Sensitive to Volatile Nitrogen Compounds
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A colorimetric paper sensor was used to detect volatile nitrogen-containing compounds emitted from spoiled salmon filets to determine their freshness. The sensing mechanism was based on acid-base reactions between acidic pH-indicating dyes and basic volatile ammonia and amines. A sensing layer was simply fabricated by drop-casting a dye solution of bromocresol green (BCG) on a polyvinylidene fluoride substrate, and its color-change response was enhanced by optimizing the amounts of additive chemicals, such as polyethylene glycol, p-toluene sulfonic acid, and graphene oxide in the dye solution. To avoid the adverse effects of water vapor, both faces of the sensing layer were enclosed by using a polyethylene terephthalate film and a gas-permeable microporous polytetrafluoroethylene sheet, respectively. When exposed to basic gas analytes, the paper-like sensor distinctly exhibited a color change from initially yellow, then to green, and finally to blue due to the deprotonation of BCG via the Brønsted acid-base reaction. The use of ammonia analyte as a test gas confirmed that the sensing performance of the optimized sensor was reversible and excellent (detection time of < 15 min, sensitive naked-eye detection at 0.25 ppm, good selectivity to common volatile organic gases, and good stability against thermal stress). Finally, the coloration intensity of the sensor was quantified as a function of the storage time of the salmon filet at 28°C to evaluate its usefulness in monitoring of the food freshness with the measurement of the total viable count (TVC) of microorganisms in the food. The TVC value increased from 3.2 × 105 to 3.1 × 109cfu/g in 28 h and then became stable, whereas the sensor response abruptly changed in the first 8 h and slightly increased thereafter. This result suggests that the colorimetric response could be used as an indicator for evaluating the degree of decay of salmon induced by microorganisms.
Abstract
A colorimetric paper sensor was used to detect volatile nitrogen-containing compounds emitted from spoiled salmon filets to determine their freshness. The sensing mechanism was based on acid-base reactions between acidic pH-indicating dyes and basic volatile ammonia and amines. A sensing layer was simply fabricated by drop-casting a dye solution of bromocresol green (BCG) on a polyvinylidene fluoride substrate, and its color-change response was enhanced by optimizing the amounts of additive chemicals, such as polyethylene glycol, p-toluene sulfonic acid, and graphene oxide in the dye solution. To avoid the adverse effects of water vapor, both faces of the sensing layer were enclosed by using a polyethylene terephthalate film and a gas-permeable microporous polytetrafluoroethylene sheet, respectively. When exposed to basic gas analytes, the paper-like sensor distinctly exhibited a color change from initially yellow, then to green, and finally to blue due to the deprotonation of BCG via the Brønsted acid-base reaction. The use of ammonia analyte as a test gas confirmed that the sensing performance of the optimized sensor was reversible and excellent (detection time of < 15 min, sensitive naked-eye detection at 0.25 ppm, good selectivity to common volatile organic gases, and good stability against thermal stress). Finally, the coloration intensity of the sensor was quantified as a function of the storage time of the salmon filet at 28°C to evaluate its usefulness in monitoring of the food freshness with the measurement of the total viable count (TVC) of microorganisms in the food. The TVC value increased from 3.2 × 105 to 3.1 × 109cfu/g in 28 h and then became stable, whereas the sensor response abruptly changed in the first 8 h and slightly increased thereafter. This result suggests that the colorimetric response could be used as an indicator for evaluating the degree of decay of salmon induced by microorganisms.
참고문헌 (18)
- D. Y. C. Fung, "Handbook of meat processing", pp.481~500 (2010), John Wiley & Sons
- H. J. Hoben, "Comparison of the pour, spread, and drop plate methods for enumeration of rhizobium spp. in inoculants made from presterilized peat", Appl. Environ. Microbiol, 44(5), pp.1246~1247 (1982)
- K. Kogure, "A tentative direct microscopic method for counting living marine bacteria", Can. J. Microbiol, 25, pp.415~420 (1979)
- P. Castro, "Total volatile base nitrogen and its use to assess freshness in European sea bass stored in ice", Food Control, 17(4), pp.245~248 (2006)
- M. Chai, "Analysis of environmental air samples by solid-phase microextraction and gas chromatography", ion trap mass spectrometry, 1995, pp.29(3), 693~701
- H. Nanto, "Zinc-oxide thin-film ammonia gas sensors with high sensitivity and excellent selectivity", J. Appl. Phys, 60(2), pp.482~484 (1986)
- 정승화, "나일론 시트와 염료를 이용한 고감도 색변환 포름알데히드 가스 센서", 센서학회지, 26(6), pp.420~426 (2017), 한국센서학회[DOI]
- R. W. Sabnis, "Handbook of acid-base indicators", pp.43~361 (2008), CRC Press
- A. Mills, "Plastic Colorimetric film sensors for gaseous ammonia", Mikrochim. Acta, 121(4), pp.225~236 (1995)
- Y. -C. Chang, "Bromocresol green", mesoporous silica adsorbent for ammonia gas sensing via an optical sensing instrument, 2011, pp.11(4), 4060~4072
- M. K. Morsy, "Development and validation of a colorimetric sensor array for fish spoilage monitoring", Food Control, 60(1), pp.346~352 (2016)
- S. L. M. Neto, "The use of euclidean geometric distance on RGB color space for the classification of sky and cloud patterns", J. Atmos. Ocean. Technol, 27(9), pp.1504~1517 (2010)
- M. Lindner, "Data-fusion of PMD-based distance-information and high-resolution RGBimages", IEEE, Int. symp. on signals circuits syst, pp.1~4 (2007)
- A. Pacquit, "Development of a smart packaging for the monitoring of fish spoilage", Food Chemistry, 102(2), pp.466~470 (2007)
- A. Pacquit, "Development of a volatile amine sensor for the monitoring of fish spoilage", Talanta, 69(2), pp.515~520 (2006)
- A. Listyarini, "A paper-based colorimetric indicator label using natural dye for monitoring shrimp spoilage", IOP Conf. Ser. : Mater. Sci. Eng, 367(1), pp.012045~ (2018)
- J. D. Buck, "The spread plate as a method for the enumeration of marine bacteria", Limnol. Oceanogr, 5(1), pp.78~80 (1960)
- A. T. Hoang, "Sensitive naked-eye detection of gaseous ammonia based on dye-impregnated nanoporous polyacrylonitrile mats", Sens. Actuator B, 230(1), pp.250~259 (2016)