알레르기 후기 반응 염증 억제 효과에 관한 太陰調胃湯의 실험적 연구
An Experimental Study on the Anti-inflammatory Effect of Taeeumjowui-tang (Taiyintiaowei-tang) in Allergic Late Inflammation
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Objective : Allergic disease, a very common chronic illness that affects all ages of patients, has been well characterized as an IgE-dependent immunologic response. Recently, interest has grown about the late inflammatory reaction as well as the early one characterized by IgE and mast cells in allergic disease. The purpose of this study was to find the anti-inflammatory effect of Taeeumjowui-tang (Taiyintiaowei-tang) in allergic reaction. Methods : The experiment was performed using Raw 264.7 cells pretreated with Taeeumjowui-tang contents extracts. The results were measured for different concentrations of Taeeumjowui-tang extracts (100, 200, 300 μg/ml): the toxicity and proliferation of cells by MTT analysis, LPS-induced NO production using Griess reagent and IL-6/ TNF-α production, which is a significant criteria for diagnosing allergic reaction. Results : No toxicity of Taeeumjowui-tang (100, 200, 300 μg/ml) on Raw 264.7 cells was found after 24 hours incubation. LPS-induced NO production was reduced after treatment with Taeeumjowui-tang (100, 200, 300 μg/ml)(P<0.001). IL-6 decreased only at 100 μg/ml(P<0.05). TNF-α production decreased only at 300μg/ml, but still statistically insignificant. There was no relationship between any components of Taeeumjowui-tang alone and inhibition of NO production. Conclusions : These data suggest that Taeeumjowui-tang has anti-inflammatory effects in allergic reaction.
Abstract
Objective : Allergic disease, a very common chronic illness that affects all ages of patients, has been well characterized as an IgE-dependent immunologic response. Recently, interest has grown about the late inflammatory reaction as well as the early one characterized by IgE and mast cells in allergic disease. The purpose of this study was to find the anti-inflammatory effect of Taeeumjowui-tang (Taiyintiaowei-tang) in allergic reaction. Methods : The experiment was performed using Raw 264.7 cells pretreated with Taeeumjowui-tang contents extracts. The results were measured for different concentrations of Taeeumjowui-tang extracts (100, 200, 300 μg/ml): the toxicity and proliferation of cells by MTT analysis, LPS-induced NO production using Griess reagent and IL-6/ TNF-α production, which is a significant criteria for diagnosing allergic reaction. Results : No toxicity of Taeeumjowui-tang (100, 200, 300 μg/ml) on Raw 264.7 cells was found after 24 hours incubation. LPS-induced NO production was reduced after treatment with Taeeumjowui-tang (100, 200, 300 μg/ml)(P<0.001). IL-6 decreased only at 100 μg/ml(P<0.05). TNF-α production decreased only at 300μg/ml, but still statistically insignificant. There was no relationship between any components of Taeeumjowui-tang alone and inhibition of NO production. Conclusions : These data suggest that Taeeumjowui-tang has anti-inflammatory effects in allergic reaction.
참고문헌 (31)
- 李.濟馬, "東醫壽世保元", pp.19~ (1993), 행림출판사
- 송일병, "알기 쉬운 사상의학", pp.214~ (1993), 하나미디어
- Bradding P, "Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitic subjects. The mast cell as a source of IL-4, IL-5, and IL-6 in human allergic mucosal inflammation", J Immunol, 151, pp.3853~3865 (1993)
- Pawankar R, "Heterogeneity of mast cells and T cells in the nasal mucosa", The Journal of allergy and clinical immunology, 98, pp.S248~S262 (1996)
- 김형갑, "葛根解肌湯의 항 Allergy 및 항염증 효과", 동의생리병리학회지, 23(2), pp.381~388 (2009), 대한동의병리학회
- Dong Hee Kim, "Gamipaidok-san Possesses Antiallergic and Anti-inflammatory Activities", 동의생리병리학회지, 19(6), pp.1659~1665 (2005), 대한동의병리학회
- Frieri M, "Nitric oxide in allergic rhinitis and asthma", Allergy Asthma Proc, 19(6), pp.349~351 (1998)
- 유태원, "太陰調胃湯의 경구 투여에 의한 SD계 흰쥐의 아급성 독성시험 연구", 동의생리병리학회지, 23(4), pp.848~852 (2009), 대한동의병리학회
- 장문석, "SD계 흰쥐에 대한 太陰調胃湯의 급성 경구 독성 시험 연구", 동의생리병리학회지, 22(6), pp.1439~1443 (2008), 대한동의병리학회
- 조세왕, "太陰調胃湯이 비만유발 흰쥐의 체중감량, 혈청지질 및 UCP1 발현에 미치는 영향", 대한한의학회지, 25(2), pp.87~97 (2004), 대한한의학회
- Shin DJ, "The effect of Taeumjowetang and Herba Ephadrae on the obeset mRNA and TNF-α mRNA in obesity- induced rats", J of Sasang Const Med., 13(1), pp.79~87 (2001)
- 주입산, "淸心蓮子湯과 太陰調胃湯이 Wistar rat의 老化에 미치는 영향", 사상체질의학회지, 17(2), pp.74~84 (2005), 사상체질의학회
- 김효정, "太陰調胃湯을 복용한 太陰人ㆍ少陰人ㆍ少陽人의 반응에 대한 임상보고", 동의생리병리학회지, 22(6), pp.1600~1605 (2008), 대한동의병리학회
- 김형구, "太陰人 알레르기성 비염환자의 四象醫學的 治療效果에 관한 임상 연구", 사상체질의학회지, 18(1), pp.138~146 (2006), 사상체질의학회
- Kim JY, "An analysis of the treatment of chronic rhinitis by Sasang constitutional medicine", J of Sasang Const Med., 14(2), pp.78~89 (2002)
- Park EK, "A study on the treatment of rhinitis by Sasang constitutional medicine", J of Sasang Const Med., 13(1), pp.109~118 (2001)
- 박종수, "三子養親湯 물 抽出物이 마우스 Th1", Th2 분화 및 알레르기 염증 반응 조절에 미치는 효과, 2006, pp.20(3), 548~556[DOI]
- 김장현, "백렴의 알레르기 염증반응에 대한 억제효과", 대한본초학회지, 23(4), pp.91~101 (2008), 대한본초학회
- 김상현, "생강나무 추출물의 알레르기성 염증반응 억제 효과", 생약학회지, 40(3), pp.233~237 (2009), 한국생약학회
- Struben VM, "Nasal nitric oxide and nasal allergy", Allergy, 61(6), pp.665~670 (2006)
- 서운교, "생강 클로로포름 분획의 활성화된 腦神經膠細胞에서 염증반응 억제효과", 대한본초학회지, 23(3), pp.73~83 (2008), 대한본초학회
- 李慶雨, "編注譯解 黃帝內經靈樞", pp.329~ (2002), 여강출판사
- 염태환, "體質鍼診療提要", pp.445~ (2007), 윗고니사
- 나창수, "한의학 총강", pp.222~ (2001), 의성당
- 전국한의과대학 폐계내과학 교실, "동의폐계내과학", pp.4~ (2009), 국진기획
- 장영주, "Effect of Taeyeumjowee -tang and electroacupuncture on obesity", 대한한방비만학회지, 1(1), pp.77~83 (2001)
- 이기주, "太陰調胃湯이 白鼠의 肥滿症 및 誘導肥滿細胞에 미치는 효과", 사상체질의학회지, 8(2), pp.219~238 (1996)
- Van Cauwenberge PB, "Nasal sensitization", Allergy, 52(33), pp.7~9 (1997)
- Ghaffar O, "Interleukin-6 expression in chronic sinusitis : colocalization of gene transcripts to eosinophils, macrophages, T lymphocytes, and mast cells", Otolaryngol Head Neck Surg., 118(4), pp.504~511 (1998)
- Riccio AM, "Cytokine pattern in allergic and non-allergic chronic rhinosinusitis in asthmatic children", Clin Exp Allergy, 32, pp.422~426 (2002)
- 이재훈, "Tumor Necrosis Factor 유전자 다형성을 이용한한국인 알레르기비염환자에서의 감수성에 대한 연구", 대한이비인후과학회지 두경부외과학, 48(9), pp.1107~1110 (2005), 대한이비인후과학회