골격근의 신장성 및 단축성 근수축 후에 면역적격세포수의 변화
Changes in Immunocompetent Cell Numbers Following Eccentric and Concentric Muscle Contraction in Skeletal Muscle
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본 연구는 골격근의 신장성 및 단축성 근수축 후에 면역적격세포수의 동태를 검증하는데 그 목적을 두었다. 실험동물은 체중 30-40g(8주령)의 ICR계 수컷 생쥐 총 84마리를 신장성 근수축 집단(ECC, n=42)과 단축성 근수축 집단(CON, n=42)으로 분류하였다. 신장성 및 단축성 근수축 (30회 5세트, 각 세트간의 휴식 1분)은 전경골근의 비골신경을 통해 전기 자극을 주었다. 전경골근의 적출은 신장성 및 단축성 근수축 전(pre, n=6), 직후(IA, n=6), 1일 후(1D, n=6), 3일 후(3D, n=6), 7일 후(7D, n=6), 14일 후(14D, n=6), 그리고 28일 후(28D, n=6)에 각각 실시하였다. 양성 근섬유수 및 면역적격세포수(T, Th, Tc, 호중구, 포식세포)는 조직학적 분석을 통해 산출하였다. 양성 섬유수는 근수축 3일 및 7일 후에 CON 집단에 비해 ECC 집단에 있어서 유의하게 높게 나타났다(p<.05). Tc세포수는 근수축 1일, 3일 및 7일후에, 호중구수는 근수축 직후, 1일, 3일 및 7일후에, 포식세포수는 근수축 직후, 1일, 3일, 7일, 14일 및 28일후에 각각 CON 집단에 비해 ECC 집단에 있어서 유의하게 높게 나타났다(p<.05). 반면, T 및 Th 세포수는 시기 및 집단 간에서 유의한 차이를 보이지 않았다. 이상의 결과에 의하면 신장성 근수축은 골격근 손상 및 면역적격세포수를 크게 증가시켰다는 것이 시사되었다.
Abstract
The purpose of this study was to examine immunocompetent cell numbers following eccentric and concentric muscle contraction in skeletal muscle. Eight-week-old Institute of Cancer Research (ICR) mice (n=84) were divided to eccentric muscle contraction group (ECC, n=42) and concentric muscle contraction group (CON, n=42). The tibialis anterior muscle was stimulated via the peroneal nerve to contract either eccentrically or concentrically (30 times × 5sets, Rest for 1 minute between each set). The tibialis anterior muscle was isolated before (pre, n=6) and 1 (n=6), 3 (n=6), 7 (n=6), 14 (n=6), and 28 d (n=6) after muscle contraction. Changes in the number of positive muscle fiber and immunocompetent cells (T, Th, Tc, neutrophils, and macrophages) were calculated by the histological analysis with HE stain and Immunohistochemistry. The number of positive muscle fiber was significantly higher (p<.05) in the ECC than in the CON at 3 and 7 d after muscle contraction. The number of Tc cells was significantly higher (p<.05) in the ECC than in the CON at 1, 3, and 7 d after the contractions. The number of neutrophils was significantly higher (p<.05) in the ECC than in the CON at immediately after, 1, 3, and 7 d after the contractions. The number of macrophages was significantly higher (p<.05) in the ECC than in the CON at all time course after muscle contraction. However, Changes in the number of T and Th cells were not significantly different between time course or groups. These results suggest that eccentric muscle contraction induces greater skeletal muscle damage and immunocompetent cells.
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