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신장성 근수축 운동에 따른 손상 조직 내 면역적격세포의 변화

Changes in immunocompetent cells in damaged muscle tissue following eccentric muscle contraction exercise

이호성
한국운동생리학회|2009.05|운동과학 (Exercise Science)|제18권 제2호|pp. 183-192|DOI 10.15857/ksep.2009.18.2.183

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참고문헌

키워드

IL-6interleukin-6면역적격세포Eccentric muscle contraction exercisemmunocompetent cells신장성 근수축 운동

초록

이 연구의 목적은 신장성 근수축 운동에 따른 손상 조직 내 면역적격세포의 변화에 대해 검토하였다. 실험동물은 ICR계 수컷 흰쥐 총 126마리(8주령)를 고빈도 집단(HG, n=42), 중빈도 집단(MG, n=42), 및 저빈도 집단(LG, n=42)으로 분류하였다. 전경골근은 비골신경에 전기 자극을 통한 신장성 근수축 운동 (HG; 90회 4세트, MG; 30회 5세트, LG; 10회 5세트, 각 세트간의 휴식 1분)을 실시하였다. 전경골근의 적출은 신장성 근수축 운동 전(before, n=6) 및 직후(IA, n=6), 1일 후(1D, n=6), 3일 후(3D, n=6), 7일 후(7D, n=6), 14일 후(14D, n=6), 그리고 28일 후(28D, n=6)에 각각 실시하였다. 호중구는 3집단 모두에서 운동 전과 비교해서 운동 3일 후에 유의하게 증가하는 것으로 나타났다(p<.05). 또한 저빈도 집단(LG) 및 고빈도 집단(HG)에서는 운동 1일 후 및 7일 후에도 유의하게 증가하는 것으로 나타났다(p<.05). 포식세포 및 Tc세포는 3집단 모두에서 운동 전과 비교해서 운동 직후, 1, 3, 7, 14, 그리고 운동 28일 후에 각각 유의하게 증가하는 것으로 나타났다(p<.05). 또한 운동 3일 후의 Tc세포수는 LG과 비교해서 MG 및 HG에서 각각 유의하게 높게 나타났다(p<.05). IL-6 유전자는 LG에서는 운동 14일 후에, MG에서는 운동 3일 후에, 그리고 HG에서는 운동 7일 후에 각각 발현하는 것으로 나타났다. 이상의 결과에 의하면 신장성 근수축 운동은 손상 조직 내 면역적격세포를 증가시켰으며, IL-6 유전자도 발현된 것을 증명하였다.

Abstract

The purpose of this study was to examine immunocompetent cells in damaged muscle tissue following eccentric muscle contraction. Eight-week-old Institute of Cancer Research (ICR) mice (n=126) were divided into 3 groups: high frequency group (HG, n=42), middle frequency group (MG, n=42), and low frequency group (LG, n=42). The tibialis anterior muscle was stimulated via the peroneal nerve to contract eccentrically muscle exercise (HG; 90 times × 4 sets, MG; 30 times × 5sets, LG; 10times × 5sets, Rest for 1 minute between each set). The tibialis anterior muscle was isolated before (n=6) and 1 (n=6), 3 (n=6), 7 (n=6), 14 (n=6), and 28 d (n=6) after muscle contraction. Neutrophils were significantly increased in all groups after 3 days of muscle contraction (p<.05) compared with before of muscle contraction. Neutrophils in LG and HG were significantly increased after 1 day and 7 days of muscle contraction (p<.05) compared with before of muscle contraction. Macrophages and cytotoxic T cells were significantly increased in all groups immediately after, 1, 3, 7, 14, and 28 days of muscle contraction (p<.05) compared with before of muscle contraction. After 3 days of muscle contraction, cytotoxic T cells were significantly higher (p<.05) in the MG and HG compared with LG. In addition, IL-6 analysis showed an expression in 14 days (LG), 3days (MG), and 7 days (HG) after muscle contraction (P <.05). These results demonstrated that eccentric muscle contraction exercise increase immunocompetent cells in damaged muscle tissue, and IL-6 gene expression.

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