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8주간의 저항성 운동과 크레아틴, 류신의 투여가 흰쥐의 근 단백질 합성 및 골격근 특성에 미치는 영향

The Effect of Dietary Creatine and Leucine Supplementation on Protein Synthesis and Functional Properties of Skeletal Muscle During 8 Weeks of Resistance Exercise

박현|옥진아|전소은
국민체육진흥공단 한국스포츠과학원 (Korea Institute of Sport Science)|2014.03|체육과학연구 (Korean Journal of Sport Science)|제25권 제1호|pp. 1-9|DOI 10.24985/kjss.2014.25.1.1

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크레아틴LeucineMuscle Protein SynthesisResistance Exercise저항성 운동AktmTOR류신근 단백질 합성근 비대CreatineMuscle Hypertrophy
주제분류체육

초록

이 연구에서는 8주간의 저항성 운동 시 크레아틴, 류신의 투여가 근 단백질 합성과 근비대에 어떠한 영향을 미치는지 규명하는데 그 목적이 있다. 실험동물은 SD계 흰쥐 수컷 32마리를 사용하였으며, 대조군(CON), 운동군(E), 운동과 크레아틴 투여군(EC), 운동과 크레아틴, 류신 투여군(ECL)으로 분류하였다. 저항성 운동은 점진적으로 증가시킨 중량을 꼬리에 연결하여 사다리를 오르는 방법을 이용하였으며(주 3회), 보조제 투여는 크레아틴은 250㎎/kg, 크레아틴+류신은 250+250㎎/kg의 양을 매일 구강 투여하였다. 실험 결과, 체중은 모든 집단에서 실험 초기에 비해 유의하게 증가하였고(p<.001), CON군의 체중이 저항성 운동을 실시한 세 집단의 체중보다 유의하게 많이 나갔다(p<.05). plantaris 무게는 E군이 CON군에 비해 유의하게 증가하였으며(p<.05), FHL 근육무게는 운동을 실시한 세 집단이 CON군에 비해 유의하게 증가하였다(p<.05). 또한 E군이 EC군, ECL군보다 유의하게 증가하였다(p<.05). 최대근력은 저항성 운동을 실시한 세 집단 모두 실험 초기에 비해 유의하게 증가하였으며(p<.001), 집단 간 차이는 없었다. Phospho-Akt 발현은 보조제를 투여한 EC군과 ECL군이 유의하게 감소하였으며(p<.05), Phospho-mTOR는 유의한 차이가 없이 증가하는 경향을 보였다. 따라서 저항성 운동은 근육량의 증가와 근력 향상에 긍정적인 영향을 미치지만, 추가적으로 처치한 크레아틴과 류신의 영향은 미비한 것으로 판단된다. 그러나 세포내 수준에서 phospho-mTOR 단백질 발현이 증가하는 경향을 보임으로써 그 가능성을 확인할 수 있었고, 추후에는 단백질 합성에 관여하는 다양한 요인들을 세밀하게 관찰하는 연구가 필요할 것으로 사료된다.

Abstract

The purpose of this study was to examine the effect of dietary creatine and leucine supplementation on protein synthesis and functional properties of skeletal muscle during 8 weeks of resistance exercise. Thirty two male Sprague-Dawley rat(5 weeks old) were randomly divided into four groups(8 rats per group): sedentary control group(CON), execise group(E), exercise with creatine supplementation group(EC), exercise with creatine, leucine supplementation group(ECL). Exercise groups were trained to climb a ladder with a weight secured to their tails(3day/week). The supplementation was administrated daily through oral gavage. Creatine supplementation was given at 250㎎/㎏/day. Creatine+leucine supplementation was given at 250+250㎎/㎏/day. All exercise groups presented significantly lower epididymal fat mass than CON(p<.05). E presented significantly higher plantaris mass than CON(p<.05). All exercise groups significantly presented higher FHL mass than CON(p<.05) and E presented higher FHL mass than EC, ECL(p<.05). EC, ECL presented significantly lower expression of Akt/PKB protein than CON, E(p<.05). There was no difference in the expression of mTOR protein. The results suggest that the creatine and leucine supplementation does not promote any additional hypertrophic effect on resistance trained skeletal muscle. But, creatine and leucine supplementation might have a potential benefit on skeletal muscle hypertrophy through the activation of protein synthesis.

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