운동 강도의 차이가 고지방 식이 쥐의 골격근 내 지방 독성과 염증성 사이토카인 신호체계에 미치는 영향
Effects of different training intensities on lipotoxicity and pro-inflammatory cytokine signalling pathway of skeletal muscle in high-fat
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목적: 본 연구의 목적은 운동 강도의 차이가 골격근 내 지방 독성과 염증성 사이토카인 신호체계에 미치는 영향을 알아보는데 있다. 방법 : 본 연구는 SD 수컷 쥐 40마리로 하였으며, 집단은 1주간의 환경 적응 기간을 거친 후 무작위로 선정하여 일반 식이 집단, 고지방 식이 집단, 고지방 식이+저강도 운동 집단 (22 m/min, 60 min, 6 days/week), 고지방 식이+중강도 운동 집단 (26 m/min, 51 min), 고지방 식이+고강도 운동 집단 (30 m/min, 46 min)으로 구분하였다. 4주간의 처치 후 혈중 유리 지방산, 골격근 내 중성지방, TNF-α, IKKα, IKKβ, p-IKKα/βser176/180, IκBα의 발현량을 측정하였다. 결과: 4주간의 처치 후 혈중 유리 지방산과 근육 내 중성지방 함량은 고지방 식이 집단에 비해 세 운동 집단에서 유의하게 감소한 것으로 나타났다 (P<0.05). 근육 내 TNF-α, IKKα, IKKβ and p-IKKα/βser176/180 단백질 발현량은 고지방 식이 집단에 비해 저강도, 중강도, 고강도 운동 집단에서 유의하게 감소한 것으로 나타났다 (P<0.05). IκBα 발현량은 고지방 식이 집단에 비해 세 운동 집단에서 유의하게 증가한 것으로 나타났다 (P<0.05). 결론: 결론적으로 4주간 고지방식이는 염증성 사이토카인 신호전달 체계를 활성화시켜 골격근의 인슐린 저항성을 증가시켰지만, 지구성 트레이닝은 고지방식이에 의한 발현되는 염증성 사이토카인 활성을 억제시켜 인슐린 저항 증가를 유의하게 완화시키는 것으로 나타났다. 반면, 운동 강도 차이에서는 고지방식이에 의한 염증성 사이토카인 발현 유의한 영향이 미치지 않은 것으로 나타났다.
Abstract
PURPOSE: The purpose of this study was to determine the effects of different training intensities on lipotoxicity and proinflammatory cytokine signal pathway of skeletal muscle in high-fat fed rats. METHODS: Forty rats were randomly divided into five groups: Normal control diet group (NC), high fat diet group (HF), high fat diet+low-intensity exercise group (HFLE, 22 m/min, 60 min, 6 days/week), high fat diet+moderate-intensity exercise group (HFME, 26 m/min, 51 min), and high fat diet+high-intensity exercise group (HFHE, 30 m/min, 46 min). After 4 weeks of high fat diet and endurance exercise training, the free fatty acid (FFA) concentrations were determined in plasma. Intramuscular triglyceride (TG), TNF-α, IKKα, IKKβ, p-IKKα/βser176/180, IκBα contents were measured in soleus muscle. RESULTS: After 4weeks treatments, Plasma FFA level and muscle TG contents were significantly lower in the three exercise (EX) groups than those in HF (P<0.05) The protein expressions of TNFα, IKKα, IKKβ and p-IKKα/βser176/180 were significantly lower in the HFLE, HFME, and HFHE groups compared to the HF group (P<0.05). Expression of IκBα in the three EX groups were significantly higher compared to that in HF (P<0.05). CONCLUSIONS: The results of this study suggested that the elevation of inflammatory cytokine protein expression due to the high-fat feeding significantly deteriorated the lipotoxicity and insulin resistance of skeletal muscle in rats, but this deterioration was significantly attenuated by aerobic training. However, the effect of different training intensities was not different in the expression of inflammatory cytokine protein in the muscle.
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