유산소 운동프로그램 참여가 소아․청소년 IDDM 환자의 항산화 유전자 발현과 지질, DNA 손상에 미치는 영향
The Effects of Aerobic Exercise Program Participation on Antioxidant Related Gene Expression and Lipid, DNA Damage in Children with IDDM
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초록
본 연구는 소아⋅청소년 IDDM 남자 환자(DM군, n=10)와 건강한 남자 어린이(HT군, n=10)를 대상으로 12주간 유산소 운동프로그램 참여 전과 후의 지질 profiles, 항산화효소 유전자 발현, 그리고 산화물 생성의 차이를 비교하는데 그 목적이 있었다. 측정 시기는 훈련 전과 훈련 후 하였으며, 신장, 체중, 체지방, WHR, 혈압, 최대산소섭취량을 측정하였고, 혈중 지질 profiles, HbA1c, ox-LDL, DNA damage, total RNA를 분석하였다. 연구결과, 체질량지수(BMI), 체지방률, 최대산소섭취량은 그룹 간, 시기 간 차이가 없었다. 이완기 혈압은 HT군에 비해 DM군이 높게 나타났으며(p<.05), TC와 HDL-c, ox-LDL은 그룹 간, 시기 간 차이가 없었다. HbA1c는 DM군이 HT군에 비해 높았지만(p<.05), 트레이닝에 의한 변화는 나타나지 않았다. 항산화 효소 유전자 발현은 방어 기전에 따라 다른 양상을 보였으며, 조직손상과의 관계도 일관성을 보여주지 않았다. DNA damage는 트레이닝 후 DM군에서 유의한 증가가 나타났다(p<.05). 따라서, 항산화 방어역량이 약한 IDDM 환자에게 운동은 일시적인 DNA damage를 수반한다고 보지만, 트레이닝에 의해 일부 항산화 효소 유전자 발현이 증대될 수 있으며, 당화 Hb 개선을 위해선 역치수준 이상의 운동강도와 더불어 무산소성 트레이닝이 필요할 것으로 사료된다.
Abstract
The purpose of this study was to compare the differences between before and after 12 week aerobic training on lipid profiles, antioxidant enzyme gene expressions, and oxidative products in children with IDDM. We therefore studied 20 children (age, 11.9±1.85 years; 10 boys) with IDDM and 10 age-matched healthy controls during 12 week of moderate intense running exercise. Blood samples for lipid profiles, HbA1c, ox-LDL, 8-OHdG, and total RNA were drawn before, and after training. There were no differences of BMI, body fat, and VO2max between groups and periods. Also, there were no differences of TC, HDL-c, and ox-LDL between groups and periods. In IDDM, baseline DBP, and HbA1c was higher than that of controls(p<.05). And exercise-induced DNA damage peak occurred greater than control(p<.05). Antioxidant enzyme gene expression displayed different aspect according to antioxidant defense mechanism, and there was no consistency that related to tissue damages. Therefore, during or immediately after intense exercise, temporary alterations in the tissue damage occurred in children with IDDM who are at weakened antioxidant defence capacity. However, some kinds of antioxidant enzyme gene expressions magnitude by long term training, and anaerobic exercise include in training may help to improve the normalization of glycated Hb.
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