극지미세조류의 유용성분 함량 및 항산화 활성
Antioxidant Activity and Contents of Bioactive Components in Polar Microalgae
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본 연구에서는 Fragilariopsis pseudonana, Chaetoceros neogracile, Stellarima microtrias 3종 극지미세조류의 아미노산, tocopherol 함량을 비교하고, 메탄올 추출물로부터 총폴리페놀 및 총 플라보노이드 함량을 분석함과 동시에 항산화 활성 및 신경세포 보호능을 검토하였다. Fragilariopsis pseudonana의 a-, (b+g)-, d-tocopherol 함량이 가장 높은 것으로 나타났고, 특히 Fragilariopsis pseudonana의 a-tocopherol 함량은 Chaetoceros neogracile 보다 약 2배 정도 높은 1035mg/100g인 것으로 확인되었으나, Stellarima microtria에서는 모두 검출되지 않았다. 또한 Fragilariopsis pseudonana의 추출물은 ABTS assay에 의한 항산화 활성이 가장 높았으며 총폴리페놀 함량도 가장 높았다. 한편, 신경세포 보호활성은 Chaetoceros neogracile 추출물에서만 확인되었다.
Abstract
In this study, bioactive components such as polyohenols, flavonoids and tocopherols were determined in cultured polar microalgae (Fragilariopsis pseudonana, Chaetoceros neogracile, Stellarima microtrias, Porosiara pseudodenticular). Antioxidant activity of methanol extracts of polar microalgae was also investigated. a-Tocopherol contents of Fragilariopsis pseudonana were almost two times higher than those of Chaetoceros neogracile. The antioxidant activity of methanol extracts of Fragilariopsis pseudonana determined by ABTS assay was higher than other algae. Total polyphenol contents of methanol extracts also showed a similar trend as antioxidant activity. The protective activity against oxidative damages induced by glutamate in PC 12 cells was shown in only Chaetoceros neogracile.
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