청각(Codium fragile) 추출물의 항산화성 및 생리활성
Comparison of Antioxidant and Physiological Activities of Different Solvent Extracts from Codium fragile
1
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
The present study investigated the chemical composition, and antioxidant and physiological activities of the Korean marine algae, Codium fragile. The solvent extracts from C. fragile were prepared using 70% ethanol, 80% methanol, and distilled water. Based on the general chemical composition, carbohydrate, crude protein, crude lipid, crude ash, and moisture were 74.22%, 16.73%, 0.66%, 4.39%, and 4.00%, respectively. Calcium, magnesium, sodium and potassium were the main minerals. The extraction yield range of the solvent extracts was 3.51–9.76%. The ranges of total polyphenol and flavonoid contents were approximately 10.97–13.76 mg/g and 8.00–8.69 mg/g, respectively. The ABTS [2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)] radical scavenging activity, reducing power and FRAP (ferric reducing antioxidant power) activities were the highest in the ethanol extract, while methanol extract exhibited the strongest nitrite oxide scavenging activity. On the other hand, tyrosinase, elastase, and xanthine oxidase inhibitory activities of the ethanol and methanol extracts were higher than those of the water extract. Furthermore, the ethanol extract exhibited the highest β-secretase inhibitory activity. The results indicate that C. fragile can be used as an antioxidant and a functional ingredient in food and pharmaceutical products.
Abstract
The present study investigated the chemical composition, and antioxidant and physiological activities of the Korean marine algae, Codium fragile. The solvent extracts from C. fragile were prepared using 70% ethanol, 80% methanol, and distilled water. Based on the general chemical composition, carbohydrate, crude protein, crude lipid, crude ash, and moisture were 74.22%, 16.73%, 0.66%, 4.39%, and 4.00%, respectively. Calcium, magnesium, sodium and potassium were the main minerals. The extraction yield range of the solvent extracts was 3.51–9.76%. The ranges of total polyphenol and flavonoid contents were approximately 10.97–13.76 mg/g and 8.00–8.69 mg/g, respectively. The ABTS [2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)] radical scavenging activity, reducing power and FRAP (ferric reducing antioxidant power) activities were the highest in the ethanol extract, while methanol extract exhibited the strongest nitrite oxide scavenging activity. On the other hand, tyrosinase, elastase, and xanthine oxidase inhibitory activities of the ethanol and methanol extracts were higher than those of the water extract. Furthermore, the ethanol extract exhibited the highest β-secretase inhibitory activity. The results indicate that C. fragile can be used as an antioxidant and a functional ingredient in food and pharmaceutical products.
참고문헌 (39)
- Official methods of analysis, "AOAC", Association of Official Analytical Chemists, 777~788 (1990)
- Barrantes E, "Inhibition of collagenase and metalloproteinases by aloins and aloe gel", Life Sci, 72, pp.843~850 (2003)[DOI]
- Benzie IFF, "The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay", Anal Biochem, 239, pp.70~76 (1996)[DOI]
- Boo SM, "Marine Plants from Korea", pp.22~ (2012), Junghaeng-Sa
- Development of physiological activity and health functional materials of seaweed, "Byun HG", 1~10 (2005)
- 조은경, "감태 열수 추출물의 생리활성", 생명과학회지, 20(11), pp.1675~1682 (2010), 한국생명과학회
- 조은경, "매생이 추출물의 angiotensin converting enzyme 및 α-glucosidase 활성 저해 효과", 생명과학회지, 21(6), pp.811~818 (2011), 한국생명과학회
- 최재홍, "자색 고구마추출물의 기능성 화장품 활성", Food Science and Preservation, 18(3), pp.414~422 (2011), 한국식품저장유통학회
- 최지원, "유산균 발효다시마(Saccharina japonica)를 첨가한 복분자 젤리의 이화학적 품질특성", 한국수산과학회지, 55(4), pp.408~416 (2022), 한국수산과학회[DOI]
- Do JR, "Biochemical functions of the components of traditional tea materials in Korea", Ph.D, pp.1992
- Iida K, "Potent inhibitors of tyrosinase activity and melanin biosynthesis from Rheum officinale", Planta Med, 61, pp.425~428 (1995)[DOI]
- 전은비, "청각(Codium fragile)을 첨가하여 제조한 막걸리의 발효 중 품질특성 및 항산화 효과", 한국수산과학회지, 52(3), pp.224~231 (2019), 한국수산과학회[DOI]
- 최영준, "채취시기에 따른 매생이, 가시파래 및 청각 식품 성분의 변화", 한국식품영양과학회지, 34(5), pp.687~693 (2005), 한국식품영양과학회
- 김진학, "추출방법에 따른 대황 추출물의 항산화 및 소화효소 저해 효과", Food Science and Preservation, 23(1), pp.97~103 (2016), 한국식품저장유통학회
- 김주성, "갯씀바귀(Ixeris repens) 추출물의 항산화성 및 생리활성", 한국수산과학회지, 54(5), pp.742~750 (2021), 한국수산과학회[DOI]
- 김지윤, "갯사상자(Cnidium japonicum) 추출물의 항산화성 및 생리활성", 한국수산과학회지, 55(3), pp.310~318 (2022), 한국수산과학회[DOI]
- 김민선, "추출방법에 따른 청각 및 모과 추출물의 항균활성 및 기능성", KSBB Journal, 33(2), pp.89~94 (2018), 한국생물공학회[DOI]
- Kim OK, "Inhibition of xanthine oxidase by seaweed extracts", J Korean Soc Food Sci Nutr, 25, pp.1069~1073 (1996)
- 김소정, "식용해조류 6종의 페놀성화합물 함량 및 항산화 활성", 한국산학기술학회논문지, 14(6), pp.3081~3088 (2013), 한국산학기술학회[DOI]
- 최영주, "청각추출물의 항산화 및 일산화질소 합성 저해 연구", 생명과학회지, 16(5), pp.788~793 (2006), 한국생명과학회
- Kim YT, "Enzymic properties of recombinant BACE2", Eur J Biochem, 269, pp.5668~5677 (2002)[DOI]
- Ko SY, "HPLC analysis and antioxidant effect of flavonoids extracted from citrus peels", M.S, pp.2005
- 곽충실, "한국산 5종 해조류의 항산화효과와 총 폴리페놀 함량과의 관련성", 한국식품영양과학회지, 34(8), pp.1143~1150 (2005), 한국식품영양과학회
- 곽연화, "해조류 NDF에 결합된 주요 무기질(Ca, P, Mg)의 함량 분석 및 추정 이용률", 한국식품영양과학회지, 39(7), pp.1073~1077 (2010), 한국식품영양과학회
- 권유리, "추출방법에 따른 톳 추출물의 항산화 및 생리활성 특성", Food Science and Preservation, 24(5), pp.631~637 (2017), 한국식품저장유통학회
- 이주희, "해조류 청각(Codium fragile) 에탄올 추출물의 항산화에 관한 연구", 문화기술의 융합, 5(4), pp.467~472 (2019), 국제문화기술진흥원
- Lee SH, "Current status and prospect of nutraceuticals from marine algae", Bull Food Tech, 24, pp.165~175 (2011)
- Lee SY., "Analysis of skin mechanism and an application for functional cosmetic from red bean (Phaseolus angularis) shell extract", Ph.D, pp.2014
- 이연지, "추출용매에 따른 톳(Hizikia fusiformis) 추출물의 항산화 및 생리활성 비교", 한국수산과학회지, 53(6), pp.886~893 (2020), 한국수산과학회[DOI]
- 이연지, "모래지치(Messerschmidia sibirica) 추출물의 항산화성 및 생리활성", 한국수산과학회지, 54(6), pp.938~947 (2021), 한국수산과학회[DOI]
- 이연지, "톳(Hizikia fusiformis) 분말을 첨가한 양갱의 이화학적 품질특성 및 항산화 효과", 한국수산과학회지, 53(4), pp.588~596 (2020), 한국수산과학회[DOI]
- Lym HJ, "The changes of nutritional constituents in Codium fragile with drying methods", M.S, pp.2010
- "Food Code" (2021)[URL]
- Moreno MI, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharmacol, 71, pp.109~114 (2000)[DOI]
- Oyaizu N, "(NZWxBXSB) F1 mouse. A new animal model of idiopathic thrombocytopenic purpura", J Exp Med, 167, pp.2017~2022 (1988)[DOI]
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Bio Med, 26, pp.1231~1237 (1999)[DOI]
- 서영완, "부산 연안 해조류 추출물의 항산화 활성 및 Tyrosinase 저해 활성 스크리닝", Ocean and Polar Research, 25(1), pp.129~132 (2003), 한국해양과학기술원
- Shetty K, "Prevention of vitrification aßociated with in vitro shoot culture of oregano, (Origanum vulgare) by Pseudomonas spp", J Plant Physiol, 147, pp.447~451 (1995)[DOI]
- 손현정, "해조류 추출물의 In Vitro 항치매 활성", 한국식품영양과학회지, 45(7), pp.966~972 (2016), 한국식품영양과학회