감태 추출물의 생리활성과 항산화 효과
In Vitro Antioxidant Activities and Bioactive Compound Profiles of Extracts from the Brown Alga Ecklonia cava
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목적: 본 연구는 기능성 원료인 감태의 항산화 및 생리활성을 분석하여 기능성 원료로서의 감태의 가능성을 밝히고 향후 연구방향을 제시하는데 목적이 있다. 방법: DPPH 및 ABTS 실험을 통해 라디칼 소거 활성을 분석하기 위해 in vitro 실험을 수행하였고, 감태 추출물의 총 폴리페놀 및 총 플라보노이드 함량을 분석하였다. 결과: DPPH와 ABTS는 라디칼 소거 활성이 증가한 것으로 나타났으며, 감태 100E는 가장 높은 항산화 효과를 나타냈다. 다음으로 감태 50E와 감태 D.W 시료는 농도 의존적으로 높은 항산화 활성을 나타냈으며, total polyphenol content 분석에서는 감태 100E에서 높은 항산화 함량을 보였으며, total flavonoid content 분석에서도 감태 100E가 높은 항산화 함량을 보였다. 결론: 본 감태의 in vitro 실험을 통해 추출물의 강력한 항산화 효과 및 항산화 함량을 확인하였다. 이는 건강기능식품, 기능성화장품, 천연치료제 등 다양한 산업분야에서 중요한 역할을 할 수 있는 기능성 소재로서의 가능성을 확인하였다.
Abstract
Purpose: Ecklonia cava is an edible brown alga with documented antitumor, anti-inflammatory, and antioxidant activities. This study analyzed the free radical scavenging capacities and bioactive compound profiles of E. cava extracts to provide foundational evidence for potential uses as a functional raw material in various food, health, and beauty products. Methods: The free radical scavenging activities of ethanol and distilled water extracts of E. cava were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. The total phenolic content was determined quantitatively using the Folin-Ciocalteu reagent, with gallic acid as the standard. And also, the flavonoid content was determined using aluminum chloride assay, with quercetin as the standard. Results: Extracts prepared using 100% ethanol (100 E), 50% ethanol (50 E), and distilled water (DW) all demonstrated substantial dose-dependent free radical scavenging activities, with the 100 E extract showing the highest scavenging capacity according to both DPPH and ABTS assays, followed by 50 E and DW extracts. Moreover, the 100 E extract exhibited scavenging activity roughly equivalent to the positive control ascorbic acid. The 100 E extract also possessed the highest total polyphenol and flavonoid contents. Conclusion: Ecklonia cava holds promise as a functional material for health foods, cosmetics, and natural therapeutics.
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