목단피 에탄올 추출물의 B16F10 멜라닌 생성 세포의 저해 작용과 항산화 활성
Antioxidation activity and Inhibition of B16F10 melanoma cell from Moutan Cortex from Ethanol Extracts.
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본 연구에서는 목단피를 에탄올로 추출하여 헥산, 디클로로메탄, 에칠아세테이트, 부탄올, 물 층의 5개 분획을 하여 cell실험을 통한 멜라닌 생성 세포의 사멸효과를 비교분석하였다. 총 6개의 시료로 DPPH를 이용한 항산화능과 NBT / XO 을 이용한 항산화능을 측정하였고, Tyrosinase 억제능과 B16F10 멜라닌 생성 세포의 사멸효과, MTT시험법을 이용한 세포의 독성을 측정하였다. 그 결과 에틸아세테이트와 부탄올 분획에서 우수한 항산화능을 확인 할 수 있었고, 에틸아세테이트 분획에서 Tyrosinase 저해 활성이 우수하였고, B16F10 멜라닌 생성 세포의 사멸효과는 전반적으로 효과적이었다. 따라서 목단피 에틸아세테이트 분획은 항산화 및 미백효과 면에서 기능성 화장품 천연성분으로의 사용이 우수할 것으로 사료된다.
Abstract
Moutan Cortex extracts has been reported to have radical scavenging activity. On these facts, biological activity and whitening effect were examined to evaluate a bioactivity of the extracts of Moutan Cortex Radicis by extraction conditions as a possible functional cosmetic agent material. We prepared ethanol extracts. Fractionation is hexane and dichloromethane and ethyl acetate and butanol and water. This is measured DPPH assay, NBT/XO assay, tyrosinase assay and MSH-induced melanin production in B-16 cells and MTT assay. Ethyl acetate and butanol fractionation of Moutan Cortex Radicis showed dose-dependently anti-oxidant activity in DPPH, NBT/XO assay. Also, ethyl acetate fraction significantly inhibited tyrosinase activity and MSH-stimulated melanin production in B-16 cells. Therefore, these results suggest that ethyl acetate fraction of Moutan Cortex Radicis will be developed as a functional cosmetic agent, such as excellent whitening agent.
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