울금의 항산화 및 미백효과
Anti-oxidant and Whitening Effects of Curcuma longa L.
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초록
화장품에 응용가능한 소재를 찾기 위한 목적으로 울금을 메탄올로 추출하여 헥산, 디클로로메탄, 에칠아세테이트, 부탄올, 물 층의 5개 분획을 하여 총 6개의 시료로 DPPH를 이용한 항산화능을 측정 하였고, Tyrosinase 억제능과 B16F10 멜라닌 생성 세포의 사멸효과, MTT시험법을 이용한 세포의 독성을 측정하였다. 그 결과 에틸아세테이트 분획 > 메탄올 추출 > 부탄올 분획 > 디클로로메탄 분획 > 헥산 분획 > 물 분획 순으로 항산화능을 나타내었고, 에틸아세테이트 분획은 IC50 8.01 μg/mL (ascorbic acid의 IC50 10.76 μg/mL)로 가장 우수한 항산화 활성을 나타내었다. 에틸아세테이트 분획에서 Tyrosinase 저해 활성은 에틸아세테이트의 IC50 31.40 μg/mL(ascorbic acid의 IC50 16.06 μg/mL)로 나타났으며, B16F10 멜라닌 생성 세포의 사멸효과 또한 우수하였다. 따라서 울금 에틸아세테이트 분획은 항산화 및 미백효과 면에서 기능성 화장품 천연성분으로의 사용이 우수할 것으로 사료된다.
Abstract
To search for new skin-lighting agents, the inhibitory property of Curcuma longa extract and five fractions(hexane, dichloromethane, ethylacetate, butanol, water) on melanogenesis and antioxidative activities were investigated. The inhibitory action of Curcuma longa extracts and fractions on mushroom tyrosinase was revealed. To explore the action of extracts and fractions on melanogenesis, the inhibition of tyrosinase and melanin contents were measured in B16F10 melanoma cells. Results show that ethylacetate>methanol>butanol>dichloromethane>hexane fractions of Curcuma longa has strong antioxidative activities, IC50 was 8.01 μg/mL (ascorbic acid IC50 10.76 μg/mL). Inhibition of tyrosinase activity, ethylacetate fraction of Curcuma longa was IC50 31.40 μg/mL(ascorbic acid IC50 16.06 μg/mL). Also, ethylacetate fraction of Curcuma longa reduced melanin contents in B16F10 cells. Therefore, this data indicate Curcuma longa as a potential pigmentation reagents.
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