홍삼에 함유된 프로토파낙사트리올계 전환사포닌의 피부주름 개선효과
Anti-wrinkle Effects of Converted Protopanaxatriol Prepared from Korean Red Ginseng
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
In order to study about human skin anti-wrinkle effect of red ginseng, red ginseng extracts and the fractions were tested to inhibition effect against MMP-2, -9, and -1 expression in human fibroblast cells by gelatin zymography and RT-PCR methods. The red ginseng samples are red ginseng extract extracted with hot water,crude saponin as butanol soluble fraction, major ginsenosides, protopanaxatrol conversions, protopanaxadiol conversions as the fractions fractionated by C18 revers phase resin and various proportions of aqueous acetonitrile. We found that propanaxatriol conversions fraction one of the red ginseng samples inhibited in a concentration-dependent manner against MMP-2, and -9 expression levels in human fibroblast cells irradiated by UV light. By HPLC analysis, protopanaxtriol conversions fraction consisted on ginsenosides Rk3, F4, Rg6,and Rh4. These results suggest that dehydrates of protopanaxatriol ginsenoside from red ginseng have an antiwrinkle activity through inhibition of MMP-2, and -9 expression in skin.
Abstract
In order to study about human skin anti-wrinkle effect of red ginseng, red ginseng extracts and the fractions were tested to inhibition effect against MMP-2, -9, and -1 expression in human fibroblast cells by gelatin zymography and RT-PCR methods. The red ginseng samples are red ginseng extract extracted with hot water,crude saponin as butanol soluble fraction, major ginsenosides, protopanaxatrol conversions, protopanaxadiol conversions as the fractions fractionated by C18 revers phase resin and various proportions of aqueous acetonitrile. We found that propanaxatriol conversions fraction one of the red ginseng samples inhibited in a concentration-dependent manner against MMP-2, and -9 expression levels in human fibroblast cells irradiated by UV light. By HPLC analysis, protopanaxtriol conversions fraction consisted on ginsenosides Rk3, F4, Rg6,and Rh4. These results suggest that dehydrates of protopanaxatriol ginsenoside from red ginseng have an antiwrinkle activity through inhibition of MMP-2, and -9 expression in skin.
참고문헌 (13)
- 소승호, "홍삼 생약 복합물(KTNG0345)의 피부 주름개선에 관한 작용기전", Journal of Ginseng Research, 32(1), pp.39~47 (2008), 고려인삼학회
- 이해준, "자외선 B 조사 마우스에서 피부손상에 대한 홍삼의 효과", Journal of Ginseng Research, 30(4), pp.194~198 (2006), 고려인삼학회
- Choi SW, "Signal transduction in aged skin cells", Kor. J. Invest. Dermatol, 21, pp.16~20 (1998)
- 최성원, "Epidermis Proliferative Effect of the Panax ginseng Ginsenoside Rb2", Archives of Pharmacal Research, 25(1), pp.71~76 (2002), 대한약학회
- Fisher GJ, "Molecular basis of sun-induced premature ageingand retinoid antagonism", Nature, 376, pp.335~339 (1996)
- Fisher GJ, "Pathophysiology of premature skin aging induced by ultraviolet light", N. Eng. J. Med, 337, pp.1419~1428 (1997)
- Fisher GJ, "Mechanism of photoagaing and chronological skin againg", Arch. Dermatol, 138, pp.1462~1470 (2002)
- Hu B, "Bioactive peptides", chitosan nanoparticles enhance cellular antioxidant activity of (-)epigallocatechin3gallate, 2013, 875~881
- Kang S, "Imflammation and extracellular Matrix Degradation Mediated by activated transcription factors nuclear factor-kB and activator protein-1 in inflammatory acne lesions in vivo", Am. J. pathol, 166, pp.1691~1699 (2005)
- Lee EH, "Ginsenoside F1 protects human HaCa T keratinocytes from ultraviolet-B-induced apoptosis by maintaining constant levels of Bcl-2", J. Invest. Dermatol, 121, pp.607~613 (2003)
- Park JD, "Advances in Ginseng Research", Proceedings of the 7th International Symposium on Ginseng, Kor. Soc. Ginseng, pp.115~126 (1998)
- Parrodo J, "Effect of aging on the various steps of protain synthesis: fragmentation of elongation factors 2", Free Radical Biology & Medicine, 26, pp.362~370 (1999)
- Ryu JH, "A dammarane glycoside from korean red ginseng", Phytochemistry, 44, pp.931~933 (1997)