인간진피섬유아세포에서 병풀(Centella asiatica )과 엽산(Follic aicd)을 주성분으로 하는 복합발효추출물과 Phytosphingosine-1-phosphate의 복합처리의 산화적 스트레스 보호 시너지효과
Synergetic Protective Effects against Oxidative Stresses by Cotreatment of Centella asiatica and Follic Acid-Ferment Extracts and Phytosphingosine-1-Phoaphte in Human Dermal Fibroblasts
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The present study aims to evaluate synergetic effects of co-treatment with Centella asiatica (C. asiatica )and follic acid-ferment extracts (CFFE) and phytosphingosine-1-phoaphte (PhS1P). Cosmetic effects ofeach of CFFE and PhS1P are relatively well-known. However, synergetic effects of co-treatment with CFFEand PhS1P are not understood currently. Therefore, we investigated synergetic effects of co-treatmentwith CFFE and PhS1P and how co-treatment with CFFE and PhS1P exerts its synergetic effects. First, wedetermined that co-treatment with specific-concentration of CFFE and PhS1P increased cell viability. At sameconcentration of CFFE and PhS1P, HDFs were resisted oxidative stress-dependent cell growth arrest. Inaddition, we show that co-treatment of CFFE and PhS1P repressed c-Jun N-terminal kinase (JNK) activationand JNK-mediated oxidative stress response in HDFs. And co-treatment of CFFE and PhS1P-mediatedblocking of JNK activation induced decrease of MMP1 mRNA expression. Therefore, co-treatment of CFFEand PhS1P synergetic protects oxidative-stress through repressing JNK phosphorylation and transcriptionalactivity of AP-1 complex. And these results suggest that cosmetics contained with both CFFE and PhS1Pmay have more therapeutic effects against oxidative-stress-mediated skin aging and formation of wrinkle.
Abstract
The present study aims to evaluate synergetic effects of co-treatment with Centella asiatica (C. asiatica )and follic acid-ferment extracts (CFFE) and phytosphingosine-1-phoaphte (PhS1P). Cosmetic effects ofeach of CFFE and PhS1P are relatively well-known. However, synergetic effects of co-treatment with CFFEand PhS1P are not understood currently. Therefore, we investigated synergetic effects of co-treatmentwith CFFE and PhS1P and how co-treatment with CFFE and PhS1P exerts its synergetic effects. First, wedetermined that co-treatment with specific-concentration of CFFE and PhS1P increased cell viability. At sameconcentration of CFFE and PhS1P, HDFs were resisted oxidative stress-dependent cell growth arrest. Inaddition, we show that co-treatment of CFFE and PhS1P repressed c-Jun N-terminal kinase (JNK) activationand JNK-mediated oxidative stress response in HDFs. And co-treatment of CFFE and PhS1P-mediatedblocking of JNK activation induced decrease of MMP1 mRNA expression. Therefore, co-treatment of CFFEand PhS1P synergetic protects oxidative-stress through repressing JNK phosphorylation and transcriptionalactivity of AP-1 complex. And these results suggest that cosmetics contained with both CFFE and PhS1Pmay have more therapeutic effects against oxidative-stress-mediated skin aging and formation of wrinkle.
참고문헌 (17)
- 권혁년, "화장품 소재를 위한 오디 발효 추출물의 항산화 효과", 대한미용학회지, 9(3), pp.221~227 (2013), 대한미용학회
- 윤영민, "병풀과 엽산을 함유한 복합미생물 발효 추출물 및 아세틸글루타민, 니코틴산 아데닌 디뉴클레오티드 인산 복합물의 피부 세포노화 억제, 콜라겐생성 촉진, 세포재생 및 세포이동 촉진, 항염, 각질세포분화 촉진, 멜라닌생성 억제 효능", 대한피부미용학회지, 11(4), pp.675~684 (2013), 대한피부미용학회
- 이송정, "Centella asiatica 추출물을 함유한 화장품이 20대 남녀의염증성 여드름 및 홍반개선에 미치는 효과", 한국미용학회지, 16(1), pp.176~184 (2010), 한국미용학회
- 이정표, "Phytosphingosine-1-phosphate가 함유된 나노리포좀 제조 및 피부 노화 개선 효과", 대한피부미용학회지, 10(4), pp.941~948 (2012), 대한피부미용학회
- 최수빈, "미세다륜침을 이용한 병풀추출물의 피부개선효과", 석사, 건강복지학과 피부비만관리전공, pp.2013
- An IS, "Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes", Int. J. Mol. Med, 30, pp.1349~1356 (2012)
- An IS, "Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts", Int. J. Mol. Med, 30, pp.1194~1202 (2012)
- Bogoyevitch MA., "The isoform-specific functions of the c-Jun N-terminal Kinases(JNKs) : differences revealed by gene targeting", Bioessays, 28, pp.923~934 (2006)
- Brinkhaus B, "Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica", Phytomedicine, 7, pp.427~448 (2000)
- Cha HJ, "Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation", Int. J. Mol. Med, 33, pp.649~653 (2014)
- Gohil KJ, "Pharmacological Review on Centella asiatica : A potential herbal cure-all", Indian J. Pharm. Sci, 5, pp.546~556 (2010)
- 김미경, "Phytosphingosine-1-phosphate stimulates chemotacticmigration of L2071 mouse fibroblasts via pertussistoxin-sensitive G-proteins", Experimental and Molecular Medicine, 39(2), pp.185~194 (2007), 생화학분자생물학회
- Kim YJ, "entella asiatica extracts modulate hydrogen peroxide induced senescence in human dermal fibroblasts", Exp. Dermatol, 20, pp.998~1003 (2011)
- Lee JP, "Phytosphingosine-1-phosphate represses the hydrogen peroxide-induced activation of c-Jun N-terminal kinase in human dermal fibroblasts through the phosphatidylinositol 3-kinase", Akt pathway, 2012, pp.304, 673~678
- Pata MO, "Plant sphingolipids:decoding the enigma of the Sphinx", New. Phytol, 185, pp.611~630 (2010)
- Tang XL, "Asiatic acid induces colon cancer cell growth inhibition and apoptosis through mitochondrial death cascade", Biol. Pharm. Bull, 32, pp.1399~1405 (2009)
- Wang F, "Oxidative stressinduced c-Jun N-terminal kinase (JNK) activation in tendon cells upregulates MMP1 mRNA and protein expression", J. Orthop. Res, 25, pp.378~389 (2007)