인간진피섬유아세포에서 Nobiletin이 Mitogen-Activated Protein Kinases를 통한 Matrix-Metalloprotease 1 발현조절에 미치는 영향
Mitogen-Activated Protein Kinases Mediated Regulation of Matrix- Metalloprotease 1 by Nobiletin in Human Dermal Fibroblasts
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Nobiletin is widely used for the treatment of cancer and inflammation disorder. In neurocytes andmelanocytes, nobiletin exposure induced dendrite formation. Recently, nobiletin is suggested as a newwhitening agent. However, in dermis, effects of nobiletin are not understood currently. Therefore, it isimportant to determine how nobiletin exerts its therapeutic action and its effect on the anti-wrinkle. Todetermine whether MMP1 gene expression is regulated by nobiletin, we measured MMP1 mRNA expressionin nobiletein-treated human dermal fibroblasts (HDFs). And we showed that transactivation activity of TPAresponsive element (TRE) which is a major regulation promoter element and mitogen-activated proteinkinase (MAPK) activity which main regulator of AP-1 complex. As shown results, nobiletin was decreaseexpression level of MMP1 mRNA in oxidative-stressed HDFs. In addition, nobiletin repressed MAPKphosphorylation and transcriptional activity of AP-1 complex in oxidative-stressed HDFs. Therefore, nobiletinprotects oxidative-stress through repressing MAPK phosphorylation and transcriptional activity of AP-1complex. In addition, these results suggest that nobiletin is a potential cosmetic ingredient repressed antiagingand anti-wrinkle in skin.
Abstract
Nobiletin is widely used for the treatment of cancer and inflammation disorder. In neurocytes andmelanocytes, nobiletin exposure induced dendrite formation. Recently, nobiletin is suggested as a newwhitening agent. However, in dermis, effects of nobiletin are not understood currently. Therefore, it isimportant to determine how nobiletin exerts its therapeutic action and its effect on the anti-wrinkle. Todetermine whether MMP1 gene expression is regulated by nobiletin, we measured MMP1 mRNA expressionin nobiletein-treated human dermal fibroblasts (HDFs). And we showed that transactivation activity of TPAresponsive element (TRE) which is a major regulation promoter element and mitogen-activated proteinkinase (MAPK) activity which main regulator of AP-1 complex. As shown results, nobiletin was decreaseexpression level of MMP1 mRNA in oxidative-stressed HDFs. In addition, nobiletin repressed MAPKphosphorylation and transcriptional activity of AP-1 complex in oxidative-stressed HDFs. Therefore, nobiletinprotects oxidative-stress through repressing MAPK phosphorylation and transcriptional activity of AP-1complex. In addition, these results suggest that nobiletin is a potential cosmetic ingredient repressed antiagingand anti-wrinkle in skin.
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