인간진피섬유아세포에서 코코아 주요성분인 Procyanidin B1이 JNK-AP1-TRE Axis를 통한 Matrix-Metalloprotease 1 발현조절에 미치는 영향
Procyanidin B1 Regualtes Matrix-Metalloprotease 1 mRNA Expression using JNK-AP1-TRE Axis in Normal Human Dermal Fibroblasts
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초록
Cocoa extracts contain various anti-oxidant which play as anti-skin aging agents. Procyanidin B1 is one of major ingredients in cocoa. However, in dermis, effects of Procyanidin B1 are not understood currently. Therefore, we investigated whether procyanidin B1 regulated extra-cellular matrix (ECM) and exerts its therapeutic action and its effect on the anti-wrinkle. We show firstly cytotoxicity of procyanidin B1. Under 100 μg/ml procyanidin B1 show no cytotoxicity in normal human dermal fibroblasts (nHDFs). In addition, we measured matrix metalloprotease 1 (MMP1) mRNA expression in procyanidin B1 exposed nHDFs. As shown results, procyanidin B1 repressed oxidative stress induced increase of MMP1 mRNA expression. And procyanidin B1 repressed activity of TPA responsive element (TRE) which is DNA binding site of AP-1 complex which consist c-Jun and c-Fos. JNK-c-Jun axis is implicated oxidative stress-mediated MMP1 mRNA regulation. In addition, JNK is activated using phosphorylation of JNK. Therefore, we shown phosphorylation in procyanidin B1-exposed nHDFs. As shown results, procyanidin B1 decreased oxidativemediated JNK phosphorylation of JNK. Overall, our results suggest procyanidin B1 is a potential cosmetic ingredient repressed antiaging and anti-wrinkle in skin using repression of oxidative-induced MMP1 expression.
Abstract
Cocoa extracts contain various anti-oxidant which play as anti-skin aging agents. Procyanidin B1 is one of major ingredients in cocoa. However, in dermis, effects of Procyanidin B1 are not understood currently. Therefore, we investigated whether procyanidin B1 regulated extra-cellular matrix (ECM) and exerts its therapeutic action and its effect on the anti-wrinkle. We show firstly cytotoxicity of procyanidin B1. Under 100 μg/ml procyanidin B1 show no cytotoxicity in normal human dermal fibroblasts (nHDFs). In addition, we measured matrix metalloprotease 1 (MMP1) mRNA expression in procyanidin B1 exposed nHDFs. As shown results, procyanidin B1 repressed oxidative stress induced increase of MMP1 mRNA expression. And procyanidin B1 repressed activity of TPA responsive element (TRE) which is DNA binding site of AP-1 complex which consist c-Jun and c-Fos. JNK-c-Jun axis is implicated oxidative stress-mediated MMP1 mRNA regulation. In addition, JNK is activated using phosphorylation of JNK. Therefore, we shown phosphorylation in procyanidin B1-exposed nHDFs. As shown results, procyanidin B1 decreased oxidativemediated JNK phosphorylation of JNK. Overall, our results suggest procyanidin B1 is a potential cosmetic ingredient repressed antiaging and anti-wrinkle in skin using repression of oxidative-induced MMP1 expression.
참고문헌 (20)
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