국산 블루베리 착즙액의 항산화 활성 및 멜라닌생성 저해효과
Anti-oxidative and Anti-melnogenesis Effects of Buleberry Extract
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Excessive production of melanin occurs in response to UV-induced DNA damage, skin hyperpigmentation, post-inflammation and so on. There are many reports on anti-oxidant, anti-inflammation, and antimelanogenesis (whitening) activities of plant polyphenols and the relations with each activity, but little reports about blueberry (Vacciniium virgatum ) juice. We investigated here the three activities from Korean blueberry juice. Three in vitro anti-oxidant activities of 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2’-azinobis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and hydroxyl radical scavenging were evaluated. The antioxidant activities (IC50 of DPPH) of the quercetin, gallic acid, ascorbic acid, and the blueberry juice were 0.03, 0.01, 0.04, and 83.20 mg/mL, respectively. In addition, the results of ABTS test were 0.11, 0.14, 0.06, and 68.00 mg/mL, respectively. At last, hydroxyl radical scavenging activities were 0.28, 0.15, 0.11 and 91.14 mg/mL. Blueberry juice inhibited mushroom tyrosinase activity with an IC50 value of 528 μg/mL, ascorbic acid 14.4 μg/mL, and arbutin 94.3 μg/mL. Moreover, melanin biosynthesis has been decreased in a-MSH treated B16F10 melanoma cells with dose dependent manner. These results suggest that Korean blueberry juice might have potential in the development of depigmenting agents.
Abstract
Excessive production of melanin occurs in response to UV-induced DNA damage, skin hyperpigmentation, post-inflammation and so on. There are many reports on anti-oxidant, anti-inflammation, and antimelanogenesis (whitening) activities of plant polyphenols and the relations with each activity, but little reports about blueberry (Vacciniium virgatum ) juice. We investigated here the three activities from Korean blueberry juice. Three in vitro anti-oxidant activities of 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2’-azinobis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and hydroxyl radical scavenging were evaluated. The antioxidant activities (IC50 of DPPH) of the quercetin, gallic acid, ascorbic acid, and the blueberry juice were 0.03, 0.01, 0.04, and 83.20 mg/mL, respectively. In addition, the results of ABTS test were 0.11, 0.14, 0.06, and 68.00 mg/mL, respectively. At last, hydroxyl radical scavenging activities were 0.28, 0.15, 0.11 and 91.14 mg/mL. Blueberry juice inhibited mushroom tyrosinase activity with an IC50 value of 528 μg/mL, ascorbic acid 14.4 μg/mL, and arbutin 94.3 μg/mL. Moreover, melanin biosynthesis has been decreased in a-MSH treated B16F10 melanoma cells with dose dependent manner. These results suggest that Korean blueberry juice might have potential in the development of depigmenting agents.
참고문헌 (28)
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