자외선 조사에 의해 노화된 인간 섬유아세포에서 P7C3의 항노화 효능
Anti-aging Effects of P7C3 in UVA-irradiated Human Dermal Fibroblasts
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목적: 자외선은 인간의 피부에 광노화를 유발하여, 피부 탄력 감소, 주름 형성 및 상처 치유 지연과 같은 피부노화현상을 야기한다고알려져 있다. 신경보호제 후보물질로 알려진 P7C3의 화장품 원료로서의 가능성을 확인하기 위하여 얼굴 유래 인간 섬유아세포에서P7C3에 의한 collagen, type I, alpha 1 (COL1A1)과 matrix metallopeptidase 1 (MMP1)의 발현양상과 상처치유 분석법을 수행하였다. 방법: Cell counting kit-8 (CCK-8) 분석법을 통해 세포독성평가와 이후 실험에 사용될 농도를 정하였다. Real-time polymerase chain reaction (real-time PCR)을 이용하여 COL1A1과 MMP1의 mRNA 발현양상을 확인하였으며, P7C3의 상처치유능을 wound healing assay를 통해 확인하였다. 결과: 자외선에 의해 발현이 감소된 COL1A1이 P7C3에 의해 증가되었고, 자외선에 의해 발현이 증가한 MMP1은 감소되었다. 더욱이 상처치유능 실험을 통해 P7C3가 섬유아세포의 이동능력을 회복시켜 상처치유에 도움이 될 수 있음을 확인하였다. 결론: 본 연구결과를 통하여 P7C3가 노화된 피부를 개선할 수 있음을 확인하였고, 향후P7C3가 기능성 화장품 개발에 응용될 수 있는 소재로서의 가능성을 확인하기 위하여 추가적인 연구가 필요할 것이다.
Abstract
Purpose: Ultraviolet (UV) radiation, specifically ultraviolet A (UVA), is known to induce photo-aging of human skin by promoting the aging phenomenon, such as skin elasticity reduction, wrinkle formation, and retardation of wound healing. Effects of P7C3, a small molecule neuroprotective drug candidate, were studied on the collagen, type I, alpha 1 (COL1A1) and matrix metallopeptidase 1 (MMP1) production in normal human face derived dermal fibroblasts (NHDFs), as a raw material of cosmetics. Methods: Cytotoxicity of the UVA on NHDFs was determined by cell counting kit-8 (CCK-8) assay. COL1A1 and MMP1 mRNA expressions were confirmed by two types of polymerase chain reaction (PCR), reverse transcription PCR and real-time PCR. In addition, wound healing assay was performed to demonstrate migration activity in P7C3-treated cells. Results: Increased COL1A1 production was observed by real-time PCR and COL1A1-enzyme linked immunosorbent assay (ELISA). The MMP1 expression was reduced by using UVA (5 J/cm2)-treated NHDFs. Furthermore, a scratch wound healing assay showed that P7C3 enhanced the migratory properties of UVA-irradiated NHDFs. Conclusion: The effects of P7C3 in improving aging skin were demonstrated in the cell efficacy test, and P7C3 is expected to have a value as an effective anti-aging material for functional cosmetics.
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