자외선 조사에 의해 노화된 인간 섬유아세포에서 쌍발이모자반 추출물의 항노화 효능
Anti-aging Effect of Sargassum patens Extract in UVA-irradiated Human Dermal Fibroblasts
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목적: 외인성 인자 중 자외선은 피부에 광노화를 유발한다. 자외선에 의해 주름, 피부 탄력감소 및 상처 치유 지연과 같은 노화현상이 발생한다고 알려져 있다. 방법: 쌍발이모자반 추출물의 기능성 소재로서의 가능성을 확인하기 위해 인간 섬유아세포에서 쌍발이모자반 추출물에 의한 MMP1과 COL1A1 등의 발현양상과 항산화효소의 발현을 확인하였다. 결과: 자외선에 의해 발현이 증가된MMP1이 쌍발이모자반 추출물에 의해 감소되었고, 자외선에 의해발현이 감소한 COL1A1은 회복되었다. 또한 쌍발이모자반 추출물이 섬유아세포 내 항산화효소인 SOD2와 CAT의 발현을 증가시켜 항산화효과를 지님을 확인하였다. 결론: 본 연구결과를 통하여쌍발이모자반 추출물이 자외선에 의해 노화가 유발된 피부를 개선할 수 있음을 확인하였고, 향후 쌍발이모자반 추출물이 기능성 소재 개발에 응용될 수 있는 가능성을 확인하기 위하여 세포내 신호전달체계 및 쌍발이모자반의 지표물질 분석 등의 추가적인 연구가필요할 것이
Abstract
Purpose: Ultraviolet (UV) irradiation causes extrinsic aging of human skin. It is believed that UV irradiation caused aging phenomena such as skin erythema, wrinkle formation, and skin elasticity reduction. Methods: The effect of Sargassum patens extract on COL1A1 and MMP1 production in normal human dermal fibroblasts was investigated as a potential cosmetic raw material. Results: In this study, I assessed how Sargassum patens extract affected COL1A1/COL3A1/MMP1/ SODs/Catalase mRNA expression and protein production of Collagen, type I and MMP1. Quantitative real-time reverse transcription–polymerase chain reaction demonstrated that the Sargassum patens extract increased the mRNA levels of COL1A1/COL3A1/SOD2/CAT genes. Additionally, when compared to UVA-treated dermal fibroblasts, collagen and type I protein increased in Sargassum patens extract-treated conditions. Furthermore, MMP1 mRNA and secreted MMP1 protein expression were reduced by Sargassum patens extract treatment. According to these findings, Sargassum patens extract increased antiaging and antioxidative effects in dermal fibroblasts. Conclusion: Therefore, I identified the antiaging effects of Sargassum patens extract, and these findings indicated that Sargassum patens could be a pharmaceutical ingredient for skin antiaging. Based on this, I expected to conduct additional research on Sargassum patens extract for cellular mechanisms.
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