카페인산 처리를 통한 자외선B에 의해 유도된 세포 손상의 보호 효과 분석
Photoprotective Effects of Caffeic Acid against Ultraviolet-induced Damages on Human Follicle Dermal Papilla Cells
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목적: 카페인산은 식물 및 조류에 많이 발견되는 항산화 페놀산 중 하나이다. 여러 논문들을 통해 카페인산은 자외선에 의한 matrix metalloproteinase 1 발현 및 피부 발암기전을 억제한다고 보고되고 있지만, 인간 진피 모유두 세포에 대한 광보호 효과에 대해서는 연구되지 않고 있다. 방법: 카페인산이 자외선에 의한 진피 모유두 세포 손상에 어떠한 영향을 미치는지에 대해서 cell viability, ultraviolet (UV), intracellular reactive oxygen species (ROS) 및 cellular senescence detection assays를 통하여 분석하였다. 결과: 카페인산은 진피 모유두 세포에 세포독성을 유발하지 않았으며, 자외선B에 의한 세포독성을 현저하게 감소시켰다. 또한, 카페인산의 처리는 자외선B에 의해 유도되는 세포 내 ROS 생성을 농도 의존적으로 감소시켰다. 뿐만 아니라, 진피 모유두 세포에 자외선B 를 처리하기 전 카페인산의 전처리는 자외선B에 의해 유도되는 세포노화를 현저하게 억제하였다. 결론: 본 연구결과를 통해 카페인산은 진피 모유두 세포에 있어, 자외선B에 의한 세포 손상을 보호하는 효능을 가짐을 증명하였다.
Abstract
Purpose: Caffeic acid is one of anti-oxidant phenolic acids and is abundant in plants and algae. Various reports have shown that caffeic acid inhibits ultraviolet (UV)-induced matrix metalloproteinase 1 upregulation and skin carcinogenesis, however, the photoprotective effects on human follicle dermal papilla cells (HFDPCs) have not been elucidated yet. Methods: We investigated if caffeic acid inhibits UV-induced cellular damages in HFDPCs by conducting cell viability, UV protection, intracellular reactive oxygen species (ROS), and cellular senescence detection assays. Results: We found that caffeic acid had a low cytotoxicity and significantly inhibited UVB-induced cytotoxicity on HFDPCs. In addition, treatment with caffeic acid decreased UVB-mediated intracellular ROS production in a dose-dependent manner. Furthermore, UVB-induced cellular senescence, which was detected by a senescence-associated β-galactosidase (SA-β-galactosidase) assay, markedly reduced in caffeic acid treatment before UVB irradiation on HFDPCs. Conclusion: Our data indicated that caffeic acid has protective effects against UVB-induced cellular damages on HFDPCs.
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