인간진피섬유아세포에서 천궁추출물의 MMP1 발현 조절 기전
Inhibition Effect of Cnidium offiinale Makino Extracts on MMP1 Expression in Human Dermal Fibroblasts
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목적: 천궁은 산형과에 속하는 약초로써 중국이 원산지이고, 한국, 중국, 일본에서 많이 사용되어 왔다. 천궁은 진통, 항염증, 월경개선, 비타민 결핍증 억제 및 고혈압에 약으로 사용되어 왔다. 하지만 아직 피부에서의 역할은 알려지지 않았다. 때문에 본 연구에서는 천궁의 인간진피섬유아세포에서의 역할을 확인하고자 한다. 방법: 천궁에 의한 matrix metalloproteinase-1 (MMP1)의 발현변화는 quantitative real time polymerase chain reaction (qRT-PCR)로 확인하였고, 추가로 activator protein 1 (AP-1)과 c-Jun N-terminal protein kinase (JNK)의 활성은 각각 luciferase assay와 western blot을 통해서 확인하였다. 결과: 자외선에 의해서 증가된 MMP1의 발현이 천궁추출물에 의해 다시 감소되었다. 또한 자외선이 조사된 인간진피섬유아세포에서 천궁추출물은 AP-1의전사활성을 감소시키고, JNK의 인산화를 감소시키는 것으로 확인되었다. 결론: 본 논문의 연구결과를 통해서 천궁추출물은 AP-1 과 JNK 인산화를 조절함으로써 MMP1의 발현을 감소시킴을 알 수 있다. 때문에 천궁추출물은 피부의 collagen 분해하는MMP1의발현을 감소시켜 collagen의 분해를 조절할 수 있는 화장품원료로서 가능성을 가지고 있다.
Abstract
Purpose: Cnidium offiinale Makino (C. offiinale) belonged to Umbelliferae family is medical herb native to China and is extensively cultivated in Korea, China and Japan. C. offiinale has been used to oriental medicine against pain, inflmmation, menstrual disturbance, and anti-vitamin defiiency disease, and also acts as a blood pressure depressant. However, effects of C. officinale are not fully understood in dermis. Therefore, in this study, we identified effects of C. officinale in dermal fibroblast. Methods: Matrix metalloproteinase-1 (MMP1) expression of C. offiinale extract was valuated using quantitative real time polymerase chain reaction (qRT-PCR). In addition, transcriptional activity of activator protein 1 (AP-1) and c-Jun N-terminal protein kinase (JNK) activity were assayed using luciferase assay and western blot. Results: Extracts of C. offiinale decreased MMP1 expression in ultraviolet (UV)-exposed human dermal fibroblasts. Moreover, C. officinale extracts showed decrease of AP-1 transcriptional activity and phospho-JNK in UV-exposed human dermal fibroblasts. Conclusion: Our results suggest that C. officinale extracts decreased MMP1 expression using decreasing AP-1 transcriptional activity and phospho-JNK. Therefore, C. officinale extracts has potential to reduce formation of wrinkle and to use as a cosmetic ingredient.
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