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淸肺瀉肝湯의 피부 항노화 효과에 대한 연구

Studies on Skin Anti-aging Effect of Chungpyesagan-tang(CPSGT)

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초록

Objectives : This study was conducted to confirm the skin anti-aging effect of Chungpyesagan-tang(CPSGT) extract. Methods : We performed MMT assay to confirm the cytotoxicity of CPSGT. After inducing matrix metalloproteinase-1(MMP-1) with tumor necrosis factor-α(TNF-α), we investigated mRNA expression and protein secretion of MMP-1 by real-time RT-PCR and ELISA. In addition, we measured the protein expression of mitogen-activated protein kinases(MAPKs) and transcription factors by western blot. Results : CPSGT was not cytotoxic at 25-800㎍/㎖. The mRNA expression and protein secretion of MMP-1 decreased when treated with CPSGT. The protein expression of p-ERK, p-JNK, p-p38 was decreased by CPSGT. In addition, the protein expression of p-c-jun and p-NF-κB, which are transcription factors, were also decreased. Conclusion : This suggests that CPSGT can inhibit MMP-1 and thus be a potential anti-aging substance.

Abstract

Objectives : This study was conducted to confirm the skin anti-aging effect of Chungpyesagan-tang(CPSGT) extract. Methods : We performed MMT assay to confirm the cytotoxicity of CPSGT. After inducing matrix metalloproteinase-1(MMP-1) with tumor necrosis factor-α(TNF-α), we investigated mRNA expression and protein secretion of MMP-1 by real-time RT-PCR and ELISA. In addition, we measured the protein expression of mitogen-activated protein kinases(MAPKs) and transcription factors by western blot. Results : CPSGT was not cytotoxic at 25-800㎍/㎖. The mRNA expression and protein secretion of MMP-1 decreased when treated with CPSGT. The protein expression of p-ERK, p-JNK, p-p38 was decreased by CPSGT. In addition, the protein expression of p-c-jun and p-NF-κB, which are transcription factors, were also decreased. Conclusion : This suggests that CPSGT can inhibit MMP-1 and thus be a potential anti-aging substance.

참고문헌 (30)

  1. Baumann L, "Skin ageing and its treatment", J Pathol, 211(2), pp.241~251 (2007)
  2. Lee CS, "Compound K inhibits MMP-1 expression through suppression of c-Src-dependent ERK activation in TNF-α-stimulated dermal fibroblast", Exp Dermatol, 23(11), pp.819~824 (2014)
  3. 윤영민, "자외선(Ultravilet)이 피부 및 피부세포 내 신호전달체계에 미치는 영향", 아시안뷰티화장품학술지, 11(3), pp.417~426 (2013), 한국피부과학연구원
  4. Quan T, "Role of age-associated alterations of the dermal extracellular matrix microenvironment in human skin aging : a mini-review", Gerontology, 61(5), pp.427~434 (2015)
  5. Quan T, "Matrix-Degrading Metalloproteinases in Photoaging", J Investig Dermatol Symp Proc, 14(1), pp.20~24 (2009)
  6. Jian J, "Iron sensitizes keratinocytes and fibroblasts to UVA-mediated matrix metalloproteinase-1 through TNF-α and ERK activation", Exp Dermatol, 20(3), pp.249~254 (2011)
  7. Rittie L, "UV-light-induced signal cascades and skin aging", Ageing Res Rev, 1(4), pp.705~720 (2022)
  8. Pillai S, "Ultraviolet radiation and skin aging : roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation – a review", Int J Cosmet Sci, 27(1), pp.17~34 (2005)
  9. Lee JM, "Dongeuisusebowon", pp.7~138 (1986), Henglim Publishing Inc
  10. National Korean Medicine University Textbook Editing Board, "Sasang Medicine", pp.280~300 (2011), Jipmoondang
  11. 이준희, "태음인ㆍ태양인체질병증 임상진료지침: 진단 및 알고리즘", 사상체질의학회지, 27(1), pp.13~41 (2015), 사상체질의학회
  12. 전수형, "태음인체질병증 임상진료지침: 리병", 사상체질의학회지, 27(1), pp.57~70 (2015), 사상체질의학회
  13. Choi DJ, "The Clinical Efficacy of Chungpyesagan-tang on Acute Stroke", J Korean Oriental Med, 23(4), pp.9~14 (2002)
  14. 박영주, "청폐사간탕(淸肺瀉肝湯)의 혈소판 응집억제 작용에 대한 in vitro 연구", 대한한방내과학회지, 31(4), pp.714~721 (2010), 대한한방내과학회
  15. Hong SG, "Protective effects of Chungpesagan-tang against ischemia", reperfusion induced cell injury, 1999, pp.5(1), 111~117
  16. Oh YH, "The Effects of Chungpesagan-Tang Extracts on Reversible Forebrain Ischemia in Sprague-Dawley Rats", Korean J Orient Int Med, 22(2), pp.161~174 (2001)
  17. 김주희, "淸肺瀉肝湯이 생쥐의 Lipopolysaccharide 유발 關節炎에 미치는 影響", 사상체질의학회지, 14(3), pp.114~131 (2002), 사상체질의학회
  18. 김은영, "태음인 청폐사간탕이 lipase 활성저해 및 db", db mouse의 체중감량, 혈장지질, UCP 1, 2 발현에 미치는 영향, 2007, pp.19(1), 171~185[DOI]
  19. Bae JH, "Effect of Cheongpesagan-tang on the changes of the weight, plasma and UCP mRNAexpression in obese SD rats induced high fat feed", J orient Rehabil Med, 12(1), pp.133~156 (2002)
  20. Koo JS, "Effects of Chungpyesagantang on the diabetic rats induced by streptozotocin", J Korean Orient Pediatr, 11(1), pp.227~248 (1997)
  21. 전보희, "LPS로 유도된 RAW 264.7 세포에서 淸肺瀉肝湯의 항산화 효과", 한방안이비인후피부과학회지, 34(4), pp.24~36 (2021), 대한한방안이비인후피부과학회
  22. Egbert M, "The matricellular protein periostin contributes to proper collagen function and is downregulated during skin aging", J Dermatol Sci, 73(1), pp.40~48 (2014)
  23. Lee J, "Emodin inhibits TNF α-induced MMP-1 expression through suppression of activator protein-1(AP-1)", Life Sci, 79(26), pp.2480~2485 (2006)
  24. Vincenti MP, "Transcriptional regulation of collagenase(MMP-1, MMP-13)genes in arthritis : integration of complex signaling pathways for the recruitment of gene-specific transcription factors", Arthritis Res, 4(3), pp.157~164 (2002)
  25. Reunanen N, "Activation of p38α MAPK Enhances Collagenase-1(Matrix Metalloproteinase(MMP)-1)and Stromelysin-1(MMP-3)Expression by mRNA Stabilization", J Biol Chem, 277(35), pp.32360~32368 (2002)
  26. Fisher GJ, "c-Jun–dependent inhibition of cutaneous procollagen transcription following ultraviolet irradiation is reversed by all-trans retinoic acid", J Clin Invest, 106(5), pp.663~670 (2000)
  27. Fisher GJ, "Retinoic Acid Inhibits Induction of c-Jun Protein by Ultraviolet Radiation that Occurs Subsequent to Activation of Mitogen-activated Protein Kinase Pathways in Human Skin In Vivo", J Clin Invest, 101(6), pp.1432~1440 (1998)
  28. Noh EM, "Protective effects of Evodiae Fructus extract against ultraviolet-induced MMP-1 and MMP-3 expression in human dermal fibroblasts", J Herb Med, 35, pp.100586~ (2022)
  29. Bae S, "Effects of Er-Miao-San extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts", Biol Res, 48, pp.1~8 (2015)
  30. Jung YR, "Anti-Wrinkle Effect of Magnesium Lithospermate B from Salvia miltiorrhiza BUNGE : Inhibition of MMPs via NF-kB Signaling", PLoS ONE, 9(8), pp.e102689~ (2014)

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