갓(Brassica juncea L. Czern.)의 잎과 종자 추출물의 항산화, 미백 및 항주름 효능
Antioxidant Activity, Whitening and Anti-wrinkle Effects of Leaf and Seed Extracts of Brassica juncea L. Czern.
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목적: 본 연구는 갓의 잎과 종자 추출물의 생리활성 평가를 실시하여 화장품 소재로써 이용가치를 규명하고자 하였다. 방법: 생리활성 평가를 위해 갓의 잎, 종자 추출물(EtOH)의 총 폴리페놀, 총 플라보노이드 함량, DPPH radical 소거능, ABTS radical 소거능, tyrosinase 저해활성, elastase 저해활성을 측정하였다. 또한 농도에 따른 항산화 효능 및 효소 저해활성을 알아보기 위해 각 추출물의 농도(50, 100, 200 mg/mL)를 다르게 하여 상관관계를 비교분석 하였다. 결과: 갓의 잎, 종자 추출물(50, 100, 200 mg/mL)의총 폴리페놀 함량의 최대치는 각각 15.03 mg TAE/g, 27.72 mg TAE/g, 총 플라보노이드 함량은 58.84 mg QE/g, 126.75 mg QE/ g, DPPH radical 소거능은 65.26%, 83.17%, ABTS radical 소거능은 47.06%, 60.57%로 확인되었다. 항산화 관련 생리활성 평가 결과, 대체로 seed가 leaf 보다 활성이 높았으며, 모두 200 mg/mL 농도에서 가장 높은 결과를 보여 양의 상관관계임을 알 수 있었다. Tyrosinase 저해활성의 최대치는 각각 22.33%, 36.58%, elastase 저해활성은 33.45%, 40.21%였다. Leaf의 경우 농도 의존적으로tyrosinase 저해활성과 elastase 저해활성이 높아진 반면, seed의 경우 elastase 저해활성에서는 농도 의존적으로 활성이 높아졌으나tyrosinase 저해활성에서는 추출물의 농도가 일정수준 이상이 되면 오히려 활성이 낮아지는 것으로 확인되었다. 결론: 본 연구 결과로 갓(Brassica juncea L. Czern.)의 잎과 종자 추출물은 항산화 효능, tyrosinase 및 elastase 활성 억제 효능의 우수성을 규명할 수있었으며, 향후 화장품 소재로써 그 이용가치가 높을 것으로 판단된다.
Abstract
Purpose: This study is to evaluate biological activity of leaf and seed extracts of Brassica juncea L and examine their utility value as cosmetic ingredients. Methods: Total polyphenol, total flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, and inhibition activities of tyrosinase and elastase were measured to evaluate biological activity of leaf and seed extracts of Brassica juncea L. Czern.(EtOH). In addition, to investigate the antioxidant effect and enzyme inhibition activities in relation to concentrations, correlation of different concentrations of 50, 100, and 200 mg/mL of each extract were comparatively analyzed. Results: The highest total polyphenol contents of leaf and seed extracts of Brassica juncea L (50, 100, and 200 mg/mL) were 15.03 and 27.72 mg TAE/g, respectively, while the total flavonoid contents were 58.84 and 126.75 mg QE/g, respectively. The DPPH scavenging effect was confirmed as 65.26% in leaf extracts and 83.17% in seed extracts, while the ABTS radical scavenging effect was confirmed as 47.06% in leaf extracts and 60.57% in seed extracts. As a result of evaluation of biological activity analysis on antioxidant effect, the activity in seed was higher than the activity in leaf, and a positive correlation was indicated as highest activities were shown in the concentration of 200 mg/mL for both leaf and seed. The highest inhibition activities of tyrosinase were 22.33% and 36.58%, respectively, while the inhibition activities of elastase were 33.45% and 40.21%, respectively. In leaf extracts, the inhibition activities of tyrosinase and elastase increased as the concentration increased, while in seed extract, the inhibition activities of elastase increased as the concentration increased, but the inhibition activities decreased when the concentration of the extract reached above a certain level. Conclusion: The results of the study confirmed leaf and seed extracts of Brassica juncea L. Czern. have excellent antioxidant effect and inhibition effect on activities of tyrosinase and elastase, and their utility value as cosmetic ingredients would be high.
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