화장품 소재로서 갓(Brassica juncea L. Czern.)의 잎과 종자 추출물의 혼합비율 최적화
Optimization the Mixing Ratio of Leaf and Seed Extracts of Brassica juncea L. Czern. as a Cosmetic Material
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목적: 본 연구에서는 화장품 소재로서 Brassica juncea L. Czern.의 잎과 종자 추출 혼합물의 생리활성을 확인하고, 반응표면분석법(RSM)을 이용하여 최적혼합비율을 도출하고자 하였다. 방법: 항산화, 미백, 항주름 효능이 최대로 발현되는 최적혼합비를 도출하기 위해 잎(X1)과 종자(X2) 추출물의 함량을 독립변수로, 총 폴리페놀 함량, 총 플라보노이드 함량, DPPH radical 소거능, ABTS radical 소거능, tyrosinase 저해활성 및 elastase 저해활성을 종속변수로 설정하였다. 결과: 항산화, 미백, 항주름 효능이 최대로 발현되는 최적혼합비율은 잎 3.28 (4.10 mg/mL), 종자 4.21 (5.26 mg/mL)로 확인되었다. 이때의 DPPH radical 소거능은 80.01%, ABTS radical 소거능은 58.50%, tyrosinase 저해활성은 22.11%, elastase 저해활성은 53.88%로 예측되었다. 또한 LPS로 염증을 유발시킨 RAW264.7 세포에 혼합물을 처리하였을 때, 대조군 대비 약 41%의 NO 생성을 억제하였으며, 세포독성은 없는 것으로 확인되었다. 결론: 본 연구에서는 항산화, 미백, 항주름 및 항염증 효능이 있는 Brassica juncea L. Czern.의 잎과 종자 추출 혼합물의 생리활성을 확인하였으며, 이와 같은 결과를 통해 본 시료를 화장품 소재로 활용할 수 있을 것으로 판단된다.
Abstract
Purpose: This study aimed to investigate the physiological activity of mustard (Brassica juncea L. Czern.) leaf and seed extracts as a cosmetic material, and optimization the mixing ratio using response surface methodology (RSM). Methods: Optimal conditions for RSM were determined the ratio of mustard leaf (X1) and seed (X2) extracts as the independent variable, and total polyphenol content, total flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, tyrosinase inhibitory activity and elastase inhibitory activity was determined as the dependent variable. Results: Optimal mixing ratio with maximum antioxidant, whitening, and anti-wrinkle effects was determined as 3.28 (4.10 mg/mL) for leaves and 4.21 (5.26 mg/mL) for seeds. The DPPH radical and ABTS radical scavenging activities, tyrosinase and elastase inhibitory activities was predicted to be 80.01%, 58.50%, 22.11%, 53.88%, respectively. In addition, when the mixture was treated in LPS-induced RAW264.7 cells, NO production was inhibited by about 41% compared to the control group, and no cytotoxicity was observed. Conclusion: In this study, the physiological activity of optimal mixture of mustard leaf and seed extracts with antioxidant, whitening, anti-wrinkle and anti-inflammatory effects has been confirmed, this finding suggest that can use as a cosmetic material.
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