울금 추출물의 피부미용식품 소재로서의 기능성 평가
Evaluation of Curcuma longa L. Water Extracts as Beauty Food Materials in B16F10 and Human Skin Fibroblasts
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목적: 생강과에 속하는 다년생 초본식물 울금(Curcuma longa L.)에 대한 항산화 및 항암 등의 연구가 비교적 다수 수행되었으나, 피부건강과 관련한 연구는 상대적으로 미미한 실정이다. 따라서, 본 연구에서는 국내 진도산 울금 물 추출물(water extracts of Curcuma longa L., CLE)의 B16F10 세포에서 멜라닌 생성 저해 활성 및 human skin fibroblasts (HSFs)에서 피부 섬유구조관련 유전자의 발현조절에 미치는 영향을 분석하여 울금 추출물의 미용기능식품 및 화장품 소재로서의 가능성을 평가하고자 하였다. 방법: B16F10, HSFs에서 CLE의 세포독성은 thiazolyl blue tetrazolium bromide (MTT) assay로 평가하였다. B16F10 세포에서CLE의 멜라닌 생성 저해 활성은 세포의 배지에 용출된 멜라닌의 정량과 이미지 분석을 실시하였다. CLE가 HSFs 세포의 피부 섬유구조 관련 유전자의 발현에 미치는 영향은 reverse transcription-polymerase chain reaction (RT-PCR) 분석법을 통해 평가하였다. 결과: MTT assay를 통해 CLE의 농도는 B16F10 및 HSFs 세포에서 400 μg/mL 이하로 결정하였다. B16F10 세포에서 50, 100, 200, 400 μg/mL의 CLE 처리는 유의적으로 멜라닌 형성을 억제하였다. 또한 HSFs에서 10, 100, 200, 400 μg/mL의 농도로CLE를 처리한 결과 procollagen1α1, procollagen1α2, elastin의 mRNA 발현수준을 현저히 증가시켰다. 결론: 본 연구결과는 CLE를이용하여 미백 및 피부섬유관련 유전자의 상향조절로 인한 피부탄력 증진에 효과가 있음을 확인하였으며 이후 미용기능식품 및 화장품 소재로서 충분히 활용될 수 있음을 시사한다.
Abstract
Purpose: To test whether water extracts of Curcuma longa L. (CLE) have the potential as beauty food materials, the inhibitory effects of CLE on melanin accumulation in B16F10 cells and regulatory effects of CLE on the skin fibril-related genes in human skin fibroblasts (HSFs) were examined in this study. Methods: Thiazolyl blue tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of CLE in B16F10 or HSFs. Inhibitory effects of CLE on the melanin accumulation were evaluated by B16F10-eluted melanin content and image analysis. The expressions of skin fibril-related genes such as procollagen1α1, procollagen1α2, and elastin in HSFs were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) analysis. Results: Based on the cytotoxicity results, the concentrations of CLE were confirmed under 400 μg/mL in both B16F10 and HSFs. The cytotoxicity of B16F10 cells with CLE for 24 h was confirmed and CLE up to 400 μg/mL were used. The CLE from 50 to 400 μg/mL significantly inhibited melanin accumulation in B16F10 cells. The HSFs treated with CLE showed up-regulated skin fibril-related genes. Conclusion: Those results show that CLE has benefits with skin-whitening and up-regulation of skin fibril-related genes, which means it could be used as an ingredient in beauty foods and/or cosmetic materials.
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