Anti-inflammatory, Anti-aging, Antioxidant, and Anti-melanogenesis Effects of Extracts from Toona sinensis Leaf
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Background: Many researchers have developed cosmetic and medicinal ingredients using natural plant extracts to regulate and improve the skin health. We investigated Toona sinensis leaf (TSL) extract as a potential functional cosmetic ingredient by testing its pharmacological effects on skin cells. Methods and Results: The anti-inflammatory and anti-aging effects of TSL were evaluated in tumor necrosis factor (TNF)-α-stimulated human epidermal keratinocytes (HaCaT) and human dermal fibroblasts (HS68) by quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Notably, TSL antioxidant effects were assessed using 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Its anti-melanogenic activity of TSL was determined by quantifying extracellular and intracellular melanin content in α-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells. Furthemore, its inhibitory effects on mushroom tyrosinase activity was investigated. Western blotting was performed to iden- tify the anti-melanogenesis mechanism. Notably, TSL decreased interleukin (IL)-6, IL-8, and matrix metalloproteinase (MMP)-1 expression in TNF-α-stimulated HaCaT and HS68 cells. Furthermore, TSL inhibited DPPH radical activity in a concentration-dependent manner, significantly reduced α- MSH-induced intra/extracellular melanin production in B16F10 cells, and inhibited mushroom tyros- inase activity. Additionally, TSL treatment decreased protein levels of melanogenic enzymes. Conclusion: Notably, TSL demonstrated anti-inflammatory, anti-aging, antioxidant, and anti- melanogenetic effects on skin cells, confirming its protective effects on skin physiology and sug- gesting its potential as a functional cosmetic ingredient.
Abstract
Background: Many researchers have developed cosmetic and medicinal ingredients using natural plant extracts to regulate and improve the skin health. We investigated Toona sinensis leaf (TSL) extract as a potential functional cosmetic ingredient by testing its pharmacological effects on skin cells. Methods and Results: The anti-inflammatory and anti-aging effects of TSL were evaluated in tumor necrosis factor (TNF)-α-stimulated human epidermal keratinocytes (HaCaT) and human dermal fibroblasts (HS68) by quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Notably, TSL antioxidant effects were assessed using 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Its anti-melanogenic activity of TSL was determined by quantifying extracellular and intracellular melanin content in α-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells. Furthemore, its inhibitory effects on mushroom tyrosinase activity was investigated. Western blotting was performed to iden- tify the anti-melanogenesis mechanism. Notably, TSL decreased interleukin (IL)-6, IL-8, and matrix metalloproteinase (MMP)-1 expression in TNF-α-stimulated HaCaT and HS68 cells. Furthermore, TSL inhibited DPPH radical activity in a concentration-dependent manner, significantly reduced α- MSH-induced intra/extracellular melanin production in B16F10 cells, and inhibited mushroom tyros- inase activity. Additionally, TSL treatment decreased protein levels of melanogenic enzymes. Conclusion: Notably, TSL demonstrated anti-inflammatory, anti-aging, antioxidant, and anti- melanogenetic effects on skin cells, confirming its protective effects on skin physiology and sug- gesting its potential as a functional cosmetic ingredient.
참고문헌 (33)
- Ågren MS, "Tumor necrosis factor-α-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase(MMP)-1 and MMP-3 ex vivo", European Journal of Cell Biology, 94, pp.12~21 (2015)
- Bertolotto C, "Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP : pivotal role of M boxes(GTCATGTGCT)and of microphthalmia", Molecular and Cellular Biology, 18, pp.694~702 (1998)
- Blanpain C, "Epidermal stem cells of the skin", Annual Review of Cell and Developmental Biology, 22, pp.339~373 (2006)
- Chen CJ, "Toona sinensis Roem tender leaf extract inhibits SARS coronavirus replication", Journal of Ethnopharmacology, 120, pp.108~111 (2008)
- Chen Y, "Terpenoids from the seeds of Toona sinensis and their ability to attenuate high glucose-induced oxidative stress and inflammation in rat glomerular mesangial cells", Molecules, 27, pp.5784~ (2022)
- Chia YC, "Anti-neoplastic effects of gallic acid, a major component of Toona sinensis leaf extract, on oral squamous carcinoma cells", Molecules, 15, pp.8377~8389 (2010)
- Choi BY, "Targeting Wnt", β-catenin pathway for developing therapies for hair loss, 2020, pp.21, 4915~
- Dinarello CA, "Interleukin-1", Cytokine and Growth Factor Reviews, 8, pp.253~265 (1997)
- Dong XJ, "New limonoids and a dihydrobenzofuran norlignan from the roots of Toona sinensis", Molecules, 18, pp.2840~2850 (2013)
- D'Orazio J, "UV radiation and the skin", International Journal of Molecular Sciences, 14, pp.12222~12248 (2013)
- Fields GB, "Interstitial collagen catabolism", Journal of Biological Chemistry, 288, pp.8785~8793 (2013)
- Grund EM, "Tumor necrosis factor-alpha regulates inflammatory and mesenchymal responses via mitogen-activated protein kinase kinase, p38, and nuclear factor kappaB in human endometriotic epithelial cells", Molecular Pharmacology, 73, pp.1394~1404 (2008)
- Hu J, "Limonoids isolated from Toona sinensis and their radical scavenging, anti-inflammatory and cytotoxic activities", Journal of Functional Foods, 20, pp.1~9 (2016)
- Järveläinen H, "Extracellular matrix molecules : Potential targets in pharmacotherapy", Pharmacological Reviews, 61, pp.198~223 (2009)
- Jegal J, "Inhibitory effects of luteolin 7-methyl ether isolated from wikstroemia ganpi on Tnf-A", Ifn-Γ mixture-induced inflammation in human keratinocyte, 2021, pp.13, 4387~
- Jeong HR, "Leaves of Cedrela sinensis attenuate chronic unpredictable mild stress-induced depression-like behavior via regulation of hormonal and inflam-matory imbalance", Antioxidants, 11, pp.2448~ (2022)
- Kim SY, "Antibaterial activity and skin moisturizing effect of Cedrela sinensis A. Juss shoots extracts", Journal of Society of Cosmetic Scientists of Korea, 36, pp.315~321 (2010)
- Lauer-Fields JL, "Triple-helical peptide analysis of collagenolytic protease activity", Biological Chemistry, 383, pp.1095~1105 (2002)
- Lee CS, "Compound K inhibits MMP-1 expression through suppression of c-Src-dependent ERK activation in TNF-α-stimulated dermal fibroblast", Experimental Dermatology, 23, pp.819~824 (2014)
- Lee CS, "A novel adamantyl benzylbenzamide derivative, AP736, suppresses melanogenesis through the inhibition of cAMP-PKA-CREB-activated microphthalmia-associated transcription factor and tyrosinase expression", Experimental Dermatology, 22, pp.762~764 (2013)
- Lim HJ, "Anti-inflammatory activities of an extract of in vitro grown adventitious shoots of Toona sinensis in LPS-treated RAW264. 7 and Propionibacterium acnes-treated HaCaT cells", Plants(Basel), 9, pp.1701~ (2020)
- Lin HC, "5-Lipoxygenase inhibitors attenuate TNF-α-induced inflammation in human synovial fibroblasts", PLoS One, 9, pp.e107890~ (2014)
- Mehrad B, "Role of cytokines in pulmonary antimicrobial host defense", Immunologic Research, 20, pp.15~27 (1999)
- Park K, "Down-regulation of IL-6, IL-8, TNF-α and IL-1β by glucosamine in HaCaT cells, but not in the presence of TNF-α", Oncology Letters, 1, pp.289~292 (2010)
- Peng W, "Toona sinensis : a comprehensive review on its traditional usages, phytochemisty, pharmacology and toxicology", Revista Brasileira de Farmacognosia, 29, pp.111~124 (2019)
- Sadi G, "Effect of vitamin C and lipoic acid on streptozotocin-induced diabetes gene expression : mRNA and protein expressions of Cu–Zn SOD and catalase", Molecular and Cellular Biochemistry, 309, pp.109~116 (2008)
- Tada M, "Alleviation effect of arbutin on oxidative stress generated through tyrosinase reaction with L-tyrosine and L-DOPA", BMC Biochemistry, 15, pp.23~ (2014)
- Tang SC, "Topical application of glycolic acid suppresses the UVB induced IL-6, IL-8, MCP-1 and COX-2 inflammation by modulating NF-κB signaling pathway in keratinocytes and mice skin", Journal of Dermatological Science, 86, pp.238~248 (2017)
- Wang KJ, "Phenolic antioxidants from Chinese toon(fresh young leaves and shoots of Toona sinensis)", Food Chemistry, 101, pp.365~371 (2007)
- Yang CJ, "Antiproliferative effect of Toona sinensis leaf extract on non–small-cell lung cancer", Translational Research, 155, pp.305~314 (2010)
- Yasumoto KI, "Functional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family genes", Journal of Biological Chemistry, 272, pp.50~3509 (1997)
- Zhang Y, "Quercetin isolated from Toona sinensis leaves attenuates hyperglycemia and protects hepatocytes in high-carbohydrate", high-fat diet and alloxan induced experimental diabetic Mice, 2016, pp.2016, 8492780~
- Zhou Y, "Autologous activated platelet-rich plasma in hair growth : a pilot study in male androgenetic alopecia with in vitro bioactivity investigation", Journal of Cosmetic Dermatology, 20, pp.1221~1230 (2021)