기능성 소재 개발을 위한 돼지태반추출물의 경구 및 피부 동물모델 안정성 평가
Oral and Topical Safety Evaluation of Porcine Placenta Extract for the Development of the Functional Ingredient Using Animal Models
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Animal-derived placenta extract (PE) are available commercially and regularly used in various ointmentsand lotions for the purpose of improving skin properties. Although the effects of PE have been largelystudied, only a few scientific studies report the skin safety evaluations of PE derived from animals such aspig. The objective of this study was to evaluated the anti-aging potential and safety of collagen in subcriticalextract of porcine homogenate of placenta (SPE) for nutricosmetic and cosmeceutical ingredients. Theexperimental animals were divided into 6 groups, and were treated for 12 wks: positive control group (PO)treated with no-ultra violet (UV)B, negative control group (NO) treated with only UVB, experimental group1 (OSPE-1) low dosage oral application treated with SPE, experimental group 2 (OSPE-2) high dosageoral application treated with SPE, experimental group 3 (OSPE-1+FSPE-1) low dosage oral and topicalapplication treated with SPE, and experimental group 4 (OSPE-2+FSPE-2) high dosage oral and topicalapplication treated with SPE. The serum biochemical markers for liver, aspartate aminotransferase (AST),and alanine aminotransferase (ALT) were also not affected by the application of SPE at any groups. ButOSPE-2+FSPE-2 had significant (p<.05) decreased in lactate dehydrogenase (LDH) level compared thanNO. The skin safety was performed to evaluate of potential toxicity using the primary irritation test andskin sensitization test. The SPE did not show any adverse reactions such as erythema and edema onintact skin sites at primary irritation test, but on abraded sites, some experimental animals showed veryslight erythema. So, the SPE was classified as a practically non-irritating material based on the score 0.48of primary irritation index. The skin sensitization study was tested by the guinea pig maximization test(GPMT) and Freund’s complete adjuvant (FCA) with intradermal injection of SPE. The skin sensitization testshowed no skin sensitization. Based on this study, the potential for SPE a nutricosmetic and cosmeceuticalingredients were proven.
Abstract
Animal-derived placenta extract (PE) are available commercially and regularly used in various ointmentsand lotions for the purpose of improving skin properties. Although the effects of PE have been largelystudied, only a few scientific studies report the skin safety evaluations of PE derived from animals such aspig. The objective of this study was to evaluated the anti-aging potential and safety of collagen in subcriticalextract of porcine homogenate of placenta (SPE) for nutricosmetic and cosmeceutical ingredients. Theexperimental animals were divided into 6 groups, and were treated for 12 wks: positive control group (PO)treated with no-ultra violet (UV)B, negative control group (NO) treated with only UVB, experimental group1 (OSPE-1) low dosage oral application treated with SPE, experimental group 2 (OSPE-2) high dosageoral application treated with SPE, experimental group 3 (OSPE-1+FSPE-1) low dosage oral and topicalapplication treated with SPE, and experimental group 4 (OSPE-2+FSPE-2) high dosage oral and topicalapplication treated with SPE. The serum biochemical markers for liver, aspartate aminotransferase (AST),and alanine aminotransferase (ALT) were also not affected by the application of SPE at any groups. ButOSPE-2+FSPE-2 had significant (p<.05) decreased in lactate dehydrogenase (LDH) level compared thanNO. The skin safety was performed to evaluate of potential toxicity using the primary irritation test andskin sensitization test. The SPE did not show any adverse reactions such as erythema and edema onintact skin sites at primary irritation test, but on abraded sites, some experimental animals showed veryslight erythema. So, the SPE was classified as a practically non-irritating material based on the score 0.48of primary irritation index. The skin sensitization study was tested by the guinea pig maximization test(GPMT) and Freund’s complete adjuvant (FCA) with intradermal injection of SPE. The skin sensitization testshowed no skin sensitization. Based on this study, the potential for SPE a nutricosmetic and cosmeceuticalingredients were proven.
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