Effect of Hominis Placenta on cutaneous wound healing in normal and diabetic mice
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BACKGROUND/OBJECTIVES: The number of diabetic patients has recently shown a rapid increase, and delayed wound healingis a major clinical complication in diabetes. In this study, the wound healing effect of Hominis placenta (HP) treatment wasinvestigated in normal and streptozotocin-induced diabetic mice. MATERIALS/METHODS: Four full thickness wounds were created using a 4 mm biopsy punch on the dorsum. HP was injectedsubcutaneously at the middle region of the upper and lower wounds. Wounds were digitally photographed and wound sizewas measured every other day until the 14th day. Wound closure rate was analyzed using CANVAS 7SE software. Woundtissues were collected on days 2, 6, and 14 after wounding for H/E, immunohistochemistry for FGF2, and Masson's trichromestaining for collagen study. RESULTS: Significantly faster wound closure rates were observed in the HP treated group than in normal and diabetes controlmice on days 6 and 8. Treatment with HP resulted in reduced localization of inflammatory cells in wounded skin at day 6in normal mice and at day 14 in diabetic mice (P < 0.01). Expression of fibroblast growth factor (FGF) 2 showed a significantincrease in the HP treated group on day 14 in both normal (P < 0.01) and diabetic mice (P < 0.05). In addition, HP treatedgroups showed a thicker collagen layer than no treatment groups, which was remarkable on the last day, day 14, in bothnormal and diabetic mice. CONCLUSIONS: Taken together, HP treatment has a beneficial effect on acceleration of cutaneous wound healing via regulationof the entire wound healing process, including inflammation, proliferation, and remodeling.
Abstract
BACKGROUND/OBJECTIVES: The number of diabetic patients has recently shown a rapid increase, and delayed wound healingis a major clinical complication in diabetes. In this study, the wound healing effect of Hominis placenta (HP) treatment wasinvestigated in normal and streptozotocin-induced diabetic mice. MATERIALS/METHODS: Four full thickness wounds were created using a 4 mm biopsy punch on the dorsum. HP was injectedsubcutaneously at the middle region of the upper and lower wounds. Wounds were digitally photographed and wound sizewas measured every other day until the 14th day. Wound closure rate was analyzed using CANVAS 7SE software. Woundtissues were collected on days 2, 6, and 14 after wounding for H/E, immunohistochemistry for FGF2, and Masson's trichromestaining for collagen study. RESULTS: Significantly faster wound closure rates were observed in the HP treated group than in normal and diabetes controlmice on days 6 and 8. Treatment with HP resulted in reduced localization of inflammatory cells in wounded skin at day 6in normal mice and at day 14 in diabetic mice (P < 0.01). Expression of fibroblast growth factor (FGF) 2 showed a significantincrease in the HP treated group on day 14 in both normal (P < 0.01) and diabetic mice (P < 0.05). In addition, HP treatedgroups showed a thicker collagen layer than no treatment groups, which was remarkable on the last day, day 14, in bothnormal and diabetic mice. CONCLUSIONS: Taken together, HP treatment has a beneficial effect on acceleration of cutaneous wound healing via regulationof the entire wound healing process, including inflammation, proliferation, and remodeling.
참고문헌 (37)
- Morain WD, "Wound healing in diabetes mellitus", Clin Plast Surg, 17, pp.493~501 (1990)
- Hehenberger K, "Inhibited proliferation of fibroblasts derived from chronic diabetic wounds and normal dermal fibroblasts treated with high glucose is associated with increased formation of l-lactate", Wound Repair Regen, 6, pp.135~141 (1998)
- McLaughlin PJ, "Topical naltrexone accelerates full-thickness wound closure in type 1 diabetic rats by stimulating angiogenesis", Exp Biol Med (Maywood), 238, pp.733~743 (2013)
- Gurtner GC, "Wound repair and regeneration", Nature, 453, pp.314~321 (2008)
- Witte MB, "Role of nitric oxide in wound repair", Am J Surg, 183, pp.406~412 (2002)
- Diegelmann RF, "Wound healing: an overview of acute, fibrotic and delayed healing", Front Biosci, 9, pp.283~289 (2004)
- Steed DL, "Effect of extensive debridement and treatment on the healing of diabetic foot ulcers. Diabetic Ulcer Study Group", J Am Coll Surg, 183, pp.61~64 (1996)
- Firat ET, "The effects of low-level laser therapy on palatal mucoperiosteal wound healing and oxidative stress status in experimental diabetic rats", Photomed Laser Surg, 31, pp.315~321 (2013)
- Ahmed N, "Advanced glycation endproducts--role in pathology of diabetic complications", Diabetes Res Clin Pract, 67, pp.3~21 (2005)
- Li J, "Angiogenesis in wound repair: angiogenic growth factors and the extracellular matrix", Microsc Res Tech, 60, pp.107~114 (2003)
- Schweigerer L, "Capillary endothelial cells express basic fibroblast growth factor, a mitogen that promotes their own growth", Nature, 325, pp.257~259 (1987)
- Montesano R, "Basic fibroblast growth factor induces angiogenesis in vitro", Proc Natl Acad Sci U S A, 83, pp.7297~7301 (1986)
- Goldfarb G, "Human placenta for chronic leg ulcers", Lancet, 2, pp.40~ (1980)
- Jang SY, "Protective effects of hominis placenta hydrolysates on radiation enteropathy in mice", NATURAL PRODUCT RESEARCH, 25(20), pp.1988~1992 (2011), TAYLOR FRANCIS LTD
- pyreneexposed rats, "Placenta", 2010, 873~879
- De D, "Regulation of trypsin activity by peptide fraction of an aqueous extract of human placenta used as wound healer", J Cell Physiol, 226, pp.2033~2040 (2011)
- De D, "Ubiquitin-like protein from human placental extract exhibits collagenase activity", PLoS One, 8, pp.e59585~ (2013)
- Hong JW, "The Effect of Human Placenta Extract in a Wound Healing Model", Annals of Plastic Surgery, 65(1), pp.96~100 (2010)
- Shen FY, "Effectiveness of pharmacopuncture for asthma: a systematic review and meta-analysis", Evid Based Complement Alternat Med, 2011 (2011)
- Yeom MJ, "Therapeutic effects of Hominis placenta injection into an acupuncture point on the inflammatory responses in subchondral bone region of adjuvant-induced polyarthritic rat", Biol Pharm Bull, 26, pp.1472~1477 (2003)
- Seo TB, "Growth-promoting activity of Hominis Placenta extract on regenerating sciatic nerve", Acta Pharmacologica Sinica, 27(1), pp.50~58 (2006), Shanghai Institute of Materia Medica
- Lee KW, "Effects of Hominis placenta on LPS-induced cell toxicity in BV2 microglial cells", JOURNAL OF ETHNOPHARMACOLOGY, 147(2), pp.286~292 (2013), ELSEVIER IRELAND LTD
- Peplow PV, "Gene expression and release of growth factors during delayed wound healing: a review of studies in diabetic animals and possible combined laser phototherapy and growth factor treatment to enhance healing", Photomed Laser Surg, 30, pp.617~636 (2012)
- Marrotte EJ, "Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice", J Clin Invest, 120, pp.4207~4219 (2010)
- Falanga V, "Wound healing and its impairment in the diabetic foot", Lancet, 366, pp.1736~1743 (2005)
- Tsourdi E, "Current aspects in the pathophysiology and treatment of chronic wounds in diabetes mellitus", Biomed Res Int, 2013, pp.385641~ (2013)
- Maruyama K, "Decreased macrophage number and activation lead to reduced lymphatic vessel formation and contribute to impaired diabetic wound healing", Am J Pathol, 170, pp.1178~1191 (2007)
- Gibran NS, "Diminished neuropeptide levels contribute to the impaired cutaneous healing response associated with diabetes mellitus", J Surg Res, 108, pp.122~128 (2002)
- Peppa M, "Advanced glycoxidation products and impaired diabetic wound healing", Wound Repair Regen, 17, pp.461~472 (2009)
- Baum CL, "Normal cutaneous wound healing: clinical correlation with cellular and molecular events", Dermatol Surg, 31, pp.674~686 (2005)
- Roberts AB, "Transforming growth factor-beta: activity and efficacy in animal models of wound healing", Wound Repair Regen, 3, pp.408~418 (1995)
- Greenhalgh DG, "PDGF and FGF stimulate wound healing in the genetically diabetic mouse", Am J Pathol, 136, pp.1235~1246 (1990)
- Matsuda H, "Role of nerve growth factor in cutaneous wound healing: accelerating effects in normal and healing-impaired diabetic mice", J Exp Med, 187, pp.297~306 (1998)
- Yoshida S, "Recombinant hepatocyte growth factor accelerates cutaneous wound healing in a diabetic mouse model", Growth Factors, 22, pp.111~119 (2004)
- Eppley BL, "Enhancement of angiogenesis by bFGF in mandibular bone graft healing in the rabbit", J Oral Maxillofac Surg, 46, pp.391~398 (1988)
- Greenhalgh DG, "Wound healing and diabetes mellitus", Clin Plast Surg, 30, pp.37~45 (2003)
- Spanheimer RG, "Decreased collagen production in diabetic rats", Diabetes, 37, pp.371~376 (1988)