병풀(Centella asiatica ) 추출물을 이용한 20~50대 여성의 두피 홍반개선 효과

Abstract

In order to analyze the anti-inflammatory effects of Centella asiatica (C. asiatica ), which is effective inwound healing, blood circulation acceleration, fine line prevention, itchiness, and sedation, this studyanalyzed its anti-inflammatory effects in the macrophage of RAW264.7 mouse, and to find out its effectsin scalp erythema improvement through clinical study, 11 people seen with scalp erythema were selectedand conducted the study for a month from May 1~31, 2014. Study objects were divided into the experimentgroup using C. asiatica extract and comparison group not using C. asiatica and were instructed to sprayabout 2 mL twice a week, in the morning and at night, and their scalps were magnified 80 times usingmicroscope (KONG PC Camera, Bomtech, Korea) and examined before experiment, 2 weeks later, and 4weeks later. To analyze the anti-inflammatory effects of C. asiatica extract, RAW 264.7 cells were culturedin 37°C chamber at 5% CO2, and they were subcultured every 3 days to be processed for the same timewith LPS. Cytotoxicity analysis was performed to find the proper concentration of C. asiatica extract,and the inflammatory factor, LPS 0.5 μg/mL was processed at 0 mg/mL, 0.01 mg/mL, 0.1 mg/mL, 1 mg/mL when processed at 1mg/mL the C. asiatica extract displayed 1.48 times the suppressive effect andwas confirmed for anti-inflammatory effect. Clinical experiment was conducted to study the improvementeffects of C. asiatica extract on scalp erythema on the experimental group using C. asiatica extract and thecomparison group not using it, The improvement in the control group due to VES was 0.00 after 2 weeksand –6.67 after 4 weeks, which means that there was no improvement of erythema, and did not show asignificant difference with over p<.05. Experiment group displayed the improvement rate of 33.33 after 2weeks and 92.67 after 4 weeks; they displayed a great improvement of scalp erythema and displayed asignificant difference with below p<.05. C. asiatica extract if released. Such results would influence in scalpimprovement products, lead to more clinical studies using other various extracts for scalp improvements tocontribute the development and utilization of scalp care products.