저분자 콜라겐을 이용한 천연추출복합물 함유 구강붕해필름의 개발과 향장기능성 연구
Development of Oral Disintegration Film Containing Naturally Derived Compounds Using Peptide Collagen and a Study of its Functions
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목적: 본 연구는 저분자콜라겐과 천연추출복합물을 이용한 구강용해필름(이너뷰티필름)을 개발하여 in vitro 실험과 인체적용시험을통하여 향장기능성을 검증하고자 한다. 방법: 이너뷰티필름의 피부세포에서의 독성 및 procollagen생성능, elastase 및 collagenase 억제능, DPPH 라디칼 소거능, tyrosinase 억제능을 측정하여 시험관시험 및 인체적용시험을 통해 피부개선 효과를 검증하였다. 결과: 저분자콜라겐과 천연추출복합물의 혼합에 의한 피부세포독성을 확인한 결과 1000 μg/mL까지 독성은 확인할 수 없었으며 주름개선의 지표인 elastase는 125 μg/mL이상 농도에서 7.2%의 효과를 나타내었으며 collagenase는 31.3 μg/mL이상에서 16.3%의 억제 효율을 나타내었다. 피부세포를 이용한 procollagen생성은 62.5 μg/mL이상일 때 음성대조군 대비 34%의 유의적인 생성효과를보였다. 항산화 활성은 250 μg/mL, 미백은 62.5 μg/mL이상 함유되었을 때 유의적인 효과가 확인되었고 6개월간 안정성확인에서도변형이나 색도변화는 없었다. 인체적용시험에서도 이너뷰티필름의 사용 전과 후의 주름개선은 최대 32.2%, 색소잡티개선은 32.5%, 모공수축은 31.5% 그리고 피부치밀도의 개선에서 12.8%의 유의미한 결과를 확인하였다. 결론: 저분자 콜라겐 및 천연추출복합물이함유된 이너뷰티필름은 주름개선, 색소잡티개선, 모공수축, 피부치밀도개선 및 항산화, 미백에 효과가 있는 것으로 확인되어 먹는화장품 및 식품으로 개발이 가능한 것으로 사료된다.
Abstract
Purpose: In this study, we aim to study the functionality of multiple effects of peptide collagen and naturally derived compounds through in vitro experiments and human application tests. Method: Cytotoxicity of inner beauty film (IBF), pro-collagen generation ability, elastase and collagenase inhibition, DPPH radical scavenging and tyrosinase inhibition ability were measured, and skin improvement, through in vitro and human application test. Results: Cytotoxicity was not confirmed up to 1000 μg/mL. Elastase inhibition was 7.2% at a concentration of 125 μg/mL, and collagenase showed an inhibition rate of 16.3% at 31.3 μg/mL. The pro-collagen synthesis showed expression of 34%, compared with the negative control when the concentration was 62.5 μg/mL. The antioxidant activity of 250 μg/mL and whitening effect of 62.5 μg/mL are showed in the Results section, and there was no observed deformation or color change even after confirming the stability for 6 months. Even in the human clinical study, the wrinkle improvement before and after using the IBF was 32.2% at maximum, skin melanin and brown spot improvement was 32.5%, pore improvement was 31.5%, and skin density improvement was 12.8%, as shown in the Results section. Conclusion: Therefore, the IBF containing the complex of peptide collagen and naturally derived compounds has a positive effect on wrinkle improvement, skin melanin and brown spot improvement, skin pore improvement, and skin density improvement, as well as exhibiting antioxidant and whitening effects. As a result, IBF can be developed as a key material for cosmetics and foods.
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