화장품 소재로서 발아 귀리 추출물의 생리활성 특성
Physiological Characteristics of Germinated Oat (Avena sativa) Extract as Raw Material for Cosmetics
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초록
목적: 본 연구에서는 귀리의 발아 전•후에 변화하는 항산화 활성과 항염 효과에 대하여 조사, 비교함으로써 새로운 화장품 소재로서의 가능성을 알아보았다. 방법: 국내산 귀리알곡을 동결 건조한 후 95% 에탄올로 추출, 건조시켜 귀리 추출물을 획득하였다. 발아 귀리 추출물은 알곡을 20℃에서 5일간 발아시킨 뒤 동일한 방법으로 준비하였다. 이렇게 획득한 귀리 및 발아 귀리 추출물을 세포 배양에 적용하여 항산화 활성, 항염 활성, 항멜라닌 활성 그리고 항노화 활성을 측정하였다. 결과: 발아 후 귀리는 발아 전 귀리에 비해 DPPH 소거활성이 112% 증가하여 2배 이상 더 높았으며, NO 생성억제 효과는 43% 증가하였다. 발아 귀리 추출물의 경우LPS로 자극받은 RAW 264.7 세포에서 Cox-2의 발현을 35%이상, 그리고 IL1-β와 IL-6의 생성은 각각 17.5%와 22%까지 감소시키는 것으로 나타나 염증 반응 관련 인자들을 효과적으로 억제하였다. 이러한 효과는 발아 과정에서 증가하는 아베난쓰라마이드 때문인 것으로 보인다. 결론: 이상의 결과로 보아 발아 귀리는 발아 전 귀리에 비해 항산화 활성이 우수하고 높은 항염 효과를 가지는것으로 보인다. 따라서 발아 귀리는 우수한 항염 효과를 나타내는 화장품 소재로 개발이 가능하다.
Abstract
Purpose: This study evaluated the antioxidant and anti-inflammatory ability of germinated and non-germinated oat (Avena sativa) grain for developing a new cosmetic raw material. Methods: Some local oat grains were freeze-dried (cryodesiccated) and extracted using 95% ethanol to obtain a germinated oat, and the non-germinated oat extract was obtained by redrying. The oat grains were germinated at 20℃ for 5 days and extracted using the same procedure as described above. Subsequently, the extracts were applied to cultured cells to determine their antioxidant, anti-inflammatory, anti-melanogenic, and anti-aging effects. Results: For the extract of germinated oat, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity, and nitric oxide (NO) generation inhibition ability was increased to 112% and 43%, respectively, compared with the non-germinated. The RAW264.7 cells stimulated with lipopolysaccharides (LPS) revealed that treatment with the germinated oat extract could downregulate the expression of inflammatory factors, such as cyclooxygenase 2, interleukin 1beta, and interleukin 6 by 35%, 17.5%, and 22%, respectively. This enhanced anti-inflammatory effect could be as a result of increased avenanthramide during the germination period. Conclusion: Germinated oat grain extract showed an enhanced ability of antioxidant and anti-inflammatory properties. For this reason, the extract of germinated oat grain can be a good source of raw material for cosmetic industry.
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