Echinacea angustifolia 추출물의 화장품 원료로서의 항주름 및 보습효과 최인정 meeth 기업부설연구소, 서울, 한국 목적:
Anti-wrinkle and Moisturizing Activity of Echinacea angustifolia Extract as a Cosmetic ingredient
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목적: 본 연구에서는 Echinacea angustifolia (E. angustifolia) 추출물이 화장품 원료로서의 기능성을 확인하기 위해 보습 및 탄력세포실험과 임상을 통한 피부개선효과를 확인하였다. 방법: HaCaT와 CCD986sk세포에 대해 MTT assay를 통한 세포독성 분석, HaCaT에 대한 hyaluronic acid 측정 및 CCD986sk에 대한 pro collagen acid를 측정하였다. 결과: CCD986sk과 HaCaT를 이용한 세포 독성 실험결과, 200 μg/mL이하 농도에서는 세포독성이 없는 것으로 확인이 되었다. 그리고 200 μg/mL에서 hyaluronic acid, 콜라겐 모두 통계적으로 유의미한 차이가 나타났다. 한편 임상을 통한 피부개선 실험에서는 수분, 유분, 모공, 색소침착, 주름, 포르피린 영역에서 대조군에 비해 높은 수치를 나타냈었으며, 특히 보습 및 주름에서는 유의미한 차이(각각 p<0.001, p<0.01)가 나타나 E. angustifolia 추출물이 함유한 화장품이 효능이 있음을 확인할 수 있었다. 결론: 본 연구를 통하여 E. angustifolia 추출물은 보습 및피부 탄력을 개선할 수 있는 기능성 화장품 원료로서의 가능성을 확인할 수 있었다
Abstract
Purpose: Echinacea angustifolia (E. angustifolia ) extract’s usefulness as a cosmetic ingredient was examined. This study confirmed its skin improvement effects through a moisturizing and elasticity test and clinical trials. Methods: Cell ability of E. angustifolia on HaCaT and CCD986sk cells was measured by MTT assay respectively. And hyaluronic acid in HaCaT cells and pro-collagen acid productions in CCD986sk cells were measured. Results: As a result of the cytotoxicity test on CCD986sk and HaCaT cells, it was confirmed that there was no cytotoxicity at a concentration of 200 μg/mL or less. At a concentration of 200 μg/mL, both hyaluronic acid and collagen productions showed statistically significant differences. Meanwhile, the skin improvement test performed in the clinical trials revealed high scores compared to a control group and a significant difference was observed especially in moisturizing and wrinkle-care effects (p <0.001, p <0.01). Conclusion: With the potential of E. angustifolia extract, this study confirmed its promise as a cosmeceutical cosmetics ingredient.
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