감마선 조사를 통한 저분자 감 탄닌의 제조와 미백, 항산화 효능 연구
Anti-oxidant and Whitening Effect of Low Molecular Weight Kaki-tannin with Gamma irradiation
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초록
목적: 화장품의 기초 소재 개발방안 중 하나로, 국내 천연물인 청도 감을 이용하여 감 탄닌을 추출하였다. 30,000 Da의 큰 분자량을 가지는 감탄닌을 화장품에 효능 성분으로 이용 가능하도록 방사선 조사를 통하여 저분자화 하여 그 효능을 평가하였다. 방법: 감을 시료로 하여 열수추출을 통하여 고분자 감탄닌을 추출하였으며 고분자 감탄닌에 Gamma ray를 조사하여 저분자화 하고 분리 정제 하였다. 방사선이 조사된 감탄닌에서 DPPH, ABTS+ 및 Hydroxyl 라디칼 소거능을 측정하였으며, in vitro상의 tyrosinase 활성 저해 효과와 B16F10 melanoma cell에서의 멜라닌 합성 억제 효과를 관찰하였다. 또한 그 때의 멜라닌 합성 관련 단백질 발현량을 western blotting을 통하여 확인하였다. 결과: In vitro 시험 결과, 방사선이 조사된 감탄닌은 항산화 효과를 나타내었으며, tyrosinase의 활성과 melanogenesis가 억제 됨을 확인하였다. 또한 방사선이 조사된 감탄난의 시료 농도가 증가함에 따라Tyrosinase, MITF, TRP-2 발현양이 감소함을 확인하였다. 결론: 본 연구를 통하여 고분자 감탄닌에 방사선을 조사하여 저분자 감탄닌을 만들고 제조된 저분자 감탄닌이 항산화, 미백 효능을 나타내어 화장품 소재로써 이용될 잠재력이 있음을 확인였하다.
Abstract
Purpose: This study aimed to investigate the use of purified Kaki-tannin obtained from a Korean natural product, Diospyros kaki Thumb. In order to confirm the potential application of kaki-tannin to cosmetics, the efficacy was evaluated by kaki-tannin that molecular weight decreased by gamma irradiation. Methods: Kaki-tannin polymer was extracted by hydrothermal extraction from persimmon. The molecular weight of Kaki-tannin polymer reduced by gamma-ray irradiation, and then separated and purified. Further, the molecular weight of this polymer was reduced by gamma-ray irradiation; subsequently, it was separated and purified. Radical scavenging activities of this reduced molecular weight Kaki-tannin were examined by the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation, and hydroxyl radical scavenging assays. Moreover, the inhibitory effects of Kaki-tannin on tyrosinase and melanin synthesis in B16F10 melanoma cells were measured in vitro. Furthermore, the expression of tyrosinase, microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)-1, and TRP-2 during melanogenesis was confirmed by western blotting. Results: Irradiated Kaki-tannin exhibited an antioxidant effect as well as suppressed tyrosinase activity and melanogenesis. The results of this study confirmed that the expression of tyrosinase, MITF, and TRP-2 decreased as the treatment of irradiated Kaki-tannin increased. Conclusion: In the present study, to incorporate high molecular weight Kaki-tannin in cosmetics, it was first irradiated with gamma rays in order to reduce its weight. This study confirmed that Kaki-tannin irradiated by gamma-ray has antioxidant and whitening effects as well as the potential to be used as a cosmetic material.
참고문헌 (14)
- Agar N, "Melanogenesis : a photoprotective response to DNA damage?", Mutation Research, 571, pp.121~132 (2005)
- Angelo T, "Development and validation of a selective HPLCUV method for thymol determination in skin permeation experiments", Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 1022, pp.81~86 (2016)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~ (1958)
- 방승혁, "Analysis of lysosomal membrane proteins exposed to melanin in HeLa cells", 환경독성보건학회지, 31, pp.1~5 (2016), 환경독성보건학회
- Chakraborty AK, "Effect of arbutin on melanogenic proteins in human melanocytes", Pigment Cell Research, 11, pp.206~212 (1998)
- 조유진, "탄닌산 분해 효소 처리에 따른 감 추출물 유효성분 변화 및 항산화능 활성 분석", 공학기술논문지, 13(3), pp.151~156 (2020), 공학기술연구원[DOI]
- Curto EV, "Inhibitors of mammalian melanocytes tyrosinase : in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors", Biochemical Pharmacology, 57, pp.663~672 (1999)
- Del Marmol V, "Tyrosinase and related proteins in mammalian pigmentation", FEBS Letters, 381, pp.165~168 (1996)
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Research, 45, pp.1474~1478 (1985)
- Jung IY, "Analysis of innovation sources and sustainability diagnosis in the K-beauty industry", STEPI Insight, 269, pp.1~36 (2021)
- 정재헌, "빅데이타 분석을 통한 한국 제조업 산업 생태계 연구", 산업경제연구, 32(4), pp.1371~1399 (2019), 한국산업경제학회[DOI]
- 김기호, "저분자량 히알루론산의 제조 및 화장품에의 응용", 대한화장품학회지, 33(3), pp.189~196 (2007), 사단법인 대한화장품학회
- 박미혜, "감 부산물 에탄올 추출물의 항산화 및 항염증활성", 한국식품조리과학회지, 34(4), pp.335~341 (2018), 한국식품조리과학회[DOI]
- Sánchez-Ferrer A, "Tyrosinase : a comprehensive review of its mechanism", Biochimica et Biophysica acta, 1247, pp.1~11 (1995)