탄닌산 분해 효소 처리에 따른 감 추출물 유효성분 변화 및 항산화능 활성 분석
Analysis of Changes in Active Components and Antioxidant Activity of Persimmon Extract by Tannase
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초록
Persimmons (Diospyros kaki Thub.) are produced mainly in the southern regions by 188,083 tons on an area of 11,132 ha, but only about 50% of the total is processed and consumed. This study attempted to find a way to increase the value of persimmon. Upon the measurement of the antioxidant activity (DPPH) of the persimmon extract at each site, it was found that an antioxidant activity of about 40% was highest in the immature pulp of the hydrothermal and ethanol (EtOH) extracts. As a result of analyzing 14 active ingredients or phenolic compounds by LC/MS/MS, it was discovered that the content of gallic acid was generally high. Therefore, the index component was selected as gallic acid and was referred to in the experiment. Previously, it was reported that when tannin acid was decomposed, the gallic acid content increased. Meanwhile, the gallic acid content increased by 3-4 times, as a result of Tannase treatment, following the change of the active ingredient in the extract. Based on these results, it is believed that developing a product that is utilized as a functional material is possible by increasing the active ingredient through tannic acid decomposition enzyme treatment of persimmon extract.
Abstract
Persimmons (Diospyros kaki Thub.) are produced mainly in the southern regions by 188,083 tons on an area of 11,132 ha, but only about 50% of the total is processed and consumed. This study attempted to find a way to increase the value of persimmon. Upon the measurement of the antioxidant activity (DPPH) of the persimmon extract at each site, it was found that an antioxidant activity of about 40% was highest in the immature pulp of the hydrothermal and ethanol (EtOH) extracts. As a result of analyzing 14 active ingredients or phenolic compounds by LC/MS/MS, it was discovered that the content of gallic acid was generally high. Therefore, the index component was selected as gallic acid and was referred to in the experiment. Previously, it was reported that when tannin acid was decomposed, the gallic acid content increased. Meanwhile, the gallic acid content increased by 3-4 times, as a result of Tannase treatment, following the change of the active ingredient in the extract. Based on these results, it is believed that developing a product that is utilized as a functional material is possible by increasing the active ingredient through tannic acid decomposition enzyme treatment of persimmon extract.
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