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인간 피부 섬유아세포에서 메밀꿀의 항산화 효과 및 광노화 개선 효과에 관한 연구

Potential Antioxidant and Antiphotoaging Effects of Fagopyrum esculentum Honey on Human Dermal Fibroblasts

안성관|배승희|김혜빈
한국피부과학연구원 (Korea Institute of Dermatological Sciences)|2022.03|아시안뷰티화장품학술지 (Asian Journal of Beauty and Cosmetology)|제20권 제1호|pp. 43-58|DOI http://dx.doi.org/10.20402/ajbc.2021.0273

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키워드

항산화콜라겐메밀꿀기질 금속단백질분해효소인간 피부 섬유아세포荞麦蜂蜜，胶原蛋白，基质金属蛋白酶，人真皮成纤维细胞，抗氧化剂
주제분류미용

초록

목적: 본 연구는 메밀꿀(F. esculentum honey)이 인간 피부 섬유아세포(HDFs) 내의 콜라겐 및 콜라겐분해효소 발현 여부를 통해 자외선(UV) 조사에서도 손상된 인간 피부 섬유아세포에 대한 항산화 효과를 입증함으로 메밀꿀이 피부 노화와 관련하여 기능성 화장품소재으로 활용 가능성을 확인하고자 한다. 방법: 메밀꿀의 항산화 효능을 확인하기 위해 총 폴리페놀 함량과 총 플라보노이드 함량, F. esculentum honey의 DPPH radical 소거능을 이용하여 분석하였다. HDFs를 메밀꿀 처리에 따른 세포 생존율과 HDFs 대상으로 정량적 qRT-PCR분석을 통해 콜라겐과 콜라겐 분해효소의 발현량을 분석하였고, UV에 의한 세포 내 활성산소종(ROS) 정량분석을 통해 메밀꿀의 효능을 분석하였다. 또한 UV에 의해 손상된 세포 복구력과 MMP 억제 및 콜라겐 생성을 유도하는 mRNA의발현 양상을 통해 메밀꿀의 효능을 분석하였다. 결과: 메밀꿀의 항산화능을 확인하기 위해 메밀꿀의 농도별 총 폴리페놀 함량과 총플라보노이드 함량, DPPH radical 소거능을 측정한 결과 농도 의존적으로 증가하였다. 이에 세포 독성 평가를 HFDs에 진행한 결과, 세포 생존율이 90% 이상 관찰되었다. 실시간 중합효소연쇄반응 실험에서 콜라겐 생성 유전자인 COL1A1, COL3A1 mRNA의 발현은 증가되였고, MMP1과 MMP3 mRNA 발현은 감소하였다. 또한, 메밀꿀은 자외선에 의한 세포독성으로부터 세포를 보호하고, 자외선 조사에 의해 생성되는 활성산소를 감소시켰다. 자외선 조사에 의해 감소된 콜라겐 타입 COL1A1과 COL3A1의 발현은 메밀꿀처리 시 농도 의존적으로 증가하였고, 자외선 조사에 의해 증가된 MMP1과 MMP3의 발현은 메밀꿀 처리 시 농도 의존적으로 감소함으로 메밀꿀 처리 시 항산화 효과가 있음을 확인되었다. 결론: 본 연구 결과를 통해 메밀꿀이 페놀과 플라보노이드 농도가 풍부한천연 항산화제이며, 자외선에 의한 피부 세포 손상 보호 효능을 확인하여 기능성 화장품 소재로써 이용 가능성을 제하시였다.

Abstract

Purpose: The present study investigated the potential use of Fagopyrum esculentum (F. esculentum) honey as a functional skin cosmetic by examining its antioxidant and antiaging effects on human dermal fibroblasts (HDFs). Methods: Total polyphenol content, total flavonoid content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of F. esculentum honey were analyzed. HDF cell viability was examined after treatment with F. esculentum honey and expression of genes encoding collagen and matrix metalloproteinases (MMPs) was measured by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Results: Analysis of antioxidant activity showed that total polyphenol content, total flavonoid content, and DPPH radical scavenging activity of F. esculentum honey increased in a dose-dependent manner. qRT-PCR analysis revealed an increase in mRNA expression of the collagen-producing genes, COL1A1 and COL3A1 , whereas MMP1 and MMP3 expression decreased in HDFs treated with F. esculentum honey. Furthermore, F. esculentum honey protected HDFs from ultraviolet (UV) irradiationinduced cytotoxicity and reduced reactive oxygen species production. Expression of COL1A1 and COL3A1 , which was reduced by UV irradiation, was restored in a dosedependent manner, whereas expression of MMP1 and MMP3 , which was increased by UV irradiation, was reduced in a dose-dependent manner in HDFs pretreated with F. esculentum honey. Conclusion: These findings indicate that F. esculentum honey is a natural antioxidant that is enriched in phenols and flavonoids and has the potential to act as an antiaging cosmetic ingredient due to its protective effects on UV irradiation-damaged skin cells.

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