세포 모델에서 괭생이모자반 추출물의 항산화, 항염증 및 주름 개선 효능
Antioxidant, Anti-inflammatory, and Anti-wrinkle Effects of Sargassum horneri Extract in vitro
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목적: 본 연구는 괭생이모자반 추출물을 이용하여 항산화 활성, 진피섬유아세포내 항염증 및 콜라겐 생성 증대 효과를 확인 목적으로 진행되었다. 방법: 괭생이모자반 추출물의 항산화 효능을 확인하기 위하여, 추출물내 총 플라보노이드 및 총 페놀의 함량, ABTS 및 DPPH 라디칼 소거능 활성을 확인하였다. 진피섬유아세포내 괭생이모자반 추출물의 항염증 효능을 확인하기위하여 LPS 투여를 통한 염증 유도 질환 모델에 추출물 투여를 통해 염증성 cytokine인 IL-6의 mRNA 및 발현양을 측정하였으며, 그 상위 기전인 MAPK의 단백질 발현을 확인하였다. 진피섬유아세포내 괭생이모자반 추출물의 콜라겐 분해 억제능의 경우 MMP1 및 pro-collagen type 1의 발현양과 collagenase및 elastase의 억제능을 확인하였다. 결과: 괭생이모자반의 DPPH 및 ABTS 라디칼 소거능이 최고 농도인 1000 μg/mL에서 각각 86.87±12 %, 76.49±2.94 % 자유 라디칼 소거능 효과를 확인하였으며, 항산화 효능이 있는 것으로 밝혀져 있는 총 폴리페놀 및 총 플라보노이드 함량이 각각 51.62±1.945 및 79.65±4.732 mg/g이 함유되어 있음을 확인하였다. 진피섬유아세포내 함염증 효능을 확인하고자 IL-6의 발현양을 확인한 결과 대조군 대비 최대 56.8% 감소하는것을 확인하였으며, 그 상위 기전인 MAPK 또한 유의하게 감소하는 것을 확인하였다. 콜라겐 분해능 억제능은 collagenase 및 elastase의 유의적인 감소를 통해 확인하였으며, 진피섬유아내 pro-collagen type 1의 증가 및 MMP1의 감소를 통해 콜라겐의 생성이 증가되었음을 확인하였다. 결론: 괭생이모자반 추출물의 항산화, 항염증, 콜라겐 분해 억제 효능을 확인하여 기능성 화장품 소재로 가능성을 확인하였다.
Abstract
Purpose: This study mainly aimed to confirm the antioxidant activity, anti-inflammatory, and collagen production enhancement effects of the Sargassum horneri (SH) extract. Methods: The measurement of the total flavonoid and total polyphenol content, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities confirmed the antioxidant efficacy of the SH extract. The expression level of interleukin 6 (IL-6) and its upstream mechanism pathway, mitogen-activated protein kinases (MAPK), were evaluated for anti-inflammatory efficacy in human dermal fibroblast (HDF) cells. The collagenase and elastase levels were measured in terms of the ability to inhibit collagen degradation. On the contrary, collagen type 1 (COL1) and matrix metallopeptidase 1 (MMP1) were conducted to confirm the collagen production ability. Results: The DPPH and ABTS radical scavenging abilities of the SH extract were 86.87%±12% and 76.49%±2.94%, respectively, at the highest concentration of 1000 μg/mL, confirming 51.62±1.945 and 79.65±4.732 mg/g of total polyphenol and total flavonoid, respectively, which are known to have antioxidant effects. The anti-inflammatory effect of SH in HDF cells was reduced by up to 56.8% compared to the control group due to a significant decrease in MAPK. The SH extract significantly reduced collagenase and elastase, thereby inhibiting collagen degradation. On the contrary, increasing COL1 and decreasing MMP1 in HDF cells confirmed collagen production. Conclusion: Therefore, SH extract is a potential functional cosmetic through its antioxidant, anti-inflammatory, and collagen degradation inhibitory activities.
참고문헌 (33)
- Cen R, "Dermal fibroblast migration and proliferation upon wounding or lipopolysaccharide exposure is mediated by stathmin", Frontiers in Pharmacology, 12, pp.781282~ (2022)
- Corre I, "The p38 pathway, a major pleiotropic cascade that transduces stress and metastatic signals in endothelial cells", Oncotarget, 8, pp.55684~55714 (2017)
- Dias MKHM, "Sargassum horneri(Turner)C. Agardh ethanol extract attenuates fine dust-induced inflammatory responses and impaired skin barrier functions in HaCaT keratinocytes", Journal of Ethnopharmacology, 273, pp.114003~ (2021)
- Dreher D, "Role of oxygen free radicals in cancer development", European Journal of Cancer, 32, pp.30~38 (1996)
- Eklouh-Molinier C, "Investigating the relationship between changes in collagen fiber orientation during skin aging and collagen", water interactions by polarized-FTIR microimaging, 2015, pp.140, 6260~6268
- Fuller B, "Role of PGE-2 and other inflammatory mediators in skin aging and their inhibition by topical natural antiinflammatories", Cosmetics, 6, pp.6~ (2019)
- Granstein RD, "The systemic administration of gamma interferon inhibits collagen synthesis and acute inflammation in a murine skin wounding model", Journal of Investigative Dermatology, 93, pp.18~27 (1989)
- Han EJ, "Sargassum horneri as a functional food ameliorated IgE", BSA-induced mast cell activation and passive cutaneous anaphylaxis in mice, 2020, pp.18, 594~
- Jenkins G, "Molecular mechanisms of skin ageing", Mechanisms of Ageing and Development, 123, pp.801~810 (2002)
- 주예은, "'네오젠 브이 바이옴 리포좀™ (NEOGEN V.BIOME LIPOSOME™)'의 인간진피섬유아 세포 내 COL1A1 및 HAS 2 유전자 발현 증가 효능", 아시안뷰티화장품학술지, 20(4), pp.451~459 (2022), 한국피부과학연구원
- Kammeyer A, "Oxidation events and skin aging", Ageing Research Reviews, 21, pp.16~29 (2015)
- 정윤우, "대식세포에서 괭생이모자반 추출물의 MAPKs 기전 통한 면역활성 증가 효과", 한국해양바이오학회지, 15(1), pp.12~23 (2023), (사)한국해양바이오학회
- 김동섭, "저분자 콜라겐을 이용한 천연추출복합물 함유 구강붕해필름의 개발과 향장기능성 연구", 아시안뷰티화장품학술지, 18(2), pp.235~247 (2020), 한국피부과학연구원[DOI]
- Kim HJ, "PI3Kγactivation is required for LPS-induced reactive oxygen species generation in respiratory epithelial cells", Inflammation Research, 61, pp.1265~1272 (2012)
- Kim ME, "Antiinflammatory effects of ethanolic extract from Sargassum horneri(Turner)C. agardh on lipopolysaccharidestimulated macrophage activation via NF-κB pathway regulation", Immunological Investigations, 44, pp.137~146 (2015)
- Ko W, "The anti-oxidative and antineuroinflammatory effects of Sargassum horneri by heme oxygenase-1 induction in BV2 and HT22 cells", Antioxidants, 10, pp.859~ (2021)
- Landau M, "Exogenous factors in skin aging", Environmental Factors in Skin Diseases, 35, pp.1~13 (2007)
- 이병재, "괭생이모자반 기반의 자원순환형 바이오 폴리머를 혼입한 콘크리트의 내구성능", 한국건설순환자원학회 논문집, 7(4), pp.445~451 (2019), 한국건설순환자원학회[DOI]
- Lee HG, "Antioxidant potential of low molecular weight fucoidans from Sargassum autumnale against H2O2-induced oxidative stress in vitro and in zebrafish models based on molecular weight changes", Food Chemistry, 384, pp.132591~ (2022)
- Lim SW, "Understanding of skin aging and its prevention and care", The Journal of Skin Barrier Research, 4, pp.71~80 (2002)
- Madhavaraj L, "Influence of Sargassum horneri mitigating odorous gas emissions from swine manure storage facilities", Sustainability, 12, pp.7587~ (2020)
- Malemud CJ, "Matrix metalloproteinases(MMPs)in health and disease : an overview", Frontiers in Bioscience-Landmark, 11, pp.1696~1701 (2006)
- Mimić-Oka J, "Free radicals in cardiovascular diseases", Facta Universitatis, Series : Medicine and Biology, 6, pp.11~22 (1999)
- Murakami S, "The edible brown seaweed Sargassum horneri(Turner)C. Agardh ameliorates high-fat diet-induced obesity, diabetes, and hepatic steatosis in mice", Nutrients, 13, pp.551~ (2021)
- 나은주, "자외선 B에 의해 손상된 인간 각질형성세포(HaCaT)에 대한 Syringic Acid의 염증 억제 및 콜라겐 생성 효과", 아시안뷰티화장품학술지, 16(4), pp.523~531 (2018), 한국피부과학연구원[DOI]
- Perron NR, "A review of the antioxidant mechanisms of polyphenol compounds related to iron binding", Cell Biochemistry and Biophysics, 53, pp.75~100 (2009)
- Pittayapruek P, "Role of matrix metalloproteinases in photoaging and photocarcinogenesis", International Journal of Molecular Sciences, 17, pp.868~ (2016)
- Pillai S, "Ultraviolet radiation and skin aging : roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation : a review", International Journal of Cosmetic Science, 27, pp.17~34 (2005)
- Poljšak B, "Free radicals and extrinsic skin aging", Dermatology Research and Practice, 2012, pp.135206~ (2012)
- 송유석, "Fucoidan Promotes the Reconstruction of Skin Equivalents", The Korean Journal of Physiology & Pharmacology, 18(4), pp.327~331 (2014), 대한약리학회
- Sanjeewa KKA, "The protective effect of Sargassum horneri against particulate matter-induced inflammation in lung tissues of an in vivo mouse asthma model", Food and Function, 10, pp.7995~8004 (2019)
- Thornton MJ, "Estrogens and aging skin", Dermatoendocrinology, 5, pp.264~270 (2013)
- 윤진숙, "눈개승마 추출물의 항산화 및 주름개선 효과", Food Science and Preservation, 19(3), pp.393~399 (2012), 한국식품저장유통학회[DOI]