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감잎, 녹차, 회화나무 열매 추출 복합물 (BLH308)의 탈모 개선 효과

Improvement of Hair Loss by a Persimmon Leaf, Green Tea, and Sophora Fruit Extract Complex (BLH308)

조수연|이민지|정종문|이화|도문호|오수빈
한국약용작물학회|2022.08|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제30권 제4호|pp. 247-263

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참고문헌

키워드

Camellia sinensis L.Diospyros kaki Thunb.Sophora japonica L.Androgenetic AlopeciaGreen Tea ExtractPersimmon Leaf ExtractSophora Fruit ExtractHair Los

초록

Background: Persimmon leaf (Diospyros kaki), green tea (Camellia sinensis), and sophora fruit (Sophora japonica) are traditional food sources known to prevent oxidative stress and inflammation. However, research on their effect in reducing hair loss is lacking. The purpose of this study was to determine whether an extract mading using these plants, BLH308 could help reduce hair loss. Methods and Results: The antioxidant activity of BLH308 in vitro was investigated as well as its anti-inflammatory effect in RAW 264.7 cells and its effect on the expression of 5-alpha reductase Ⅱ in human dermal papilla cells. BLH308 showed significant antioxidant activity owing to its ability to scavenge 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals. Moreover, BLH308 effectively inhibited the lipopolysaccharide-induced production of interleukin-6 and tumor necrosis factor-α in RAW 264.7 cells, and downregulated the expression of 5-alpha reductase Ⅱ in human dermal papilla cells. In vivo, the hair growth promoting effect was evaluated using hair growth visual scoring. BLH308 reduced hair loss in testosterone propionateinduced hair loss C57BL/6N mice by promoting the onset of growing phase of the hair growth cycle. Conclusions: Our results showed that BLH308 can promote hair regeneration and repair hair loss, and is a potential novel candidate for the treatment of androgenetic alopecia.

Abstract

Background: Persimmon leaf (Diospyros kaki), green tea (Camellia sinensis), and sophora fruit (Sophora japonica) are traditional food sources known to prevent oxidative stress and inflammation. However, research on their effect in reducing hair loss is lacking. The purpose of this study was to determine whether an extract mading using these plants, BLH308 could help reduce hair loss. Methods and Results: The antioxidant activity of BLH308 in vitro was investigated as well as its anti-inflammatory effect in RAW 264.7 cells and its effect on the expression of 5-alpha reductase Ⅱ in human dermal papilla cells. BLH308 showed significant antioxidant activity owing to its ability to scavenge 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals. Moreover, BLH308 effectively inhibited the lipopolysaccharide-induced production of interleukin-6 and tumor necrosis factor-α in RAW 264.7 cells, and downregulated the expression of 5-alpha reductase Ⅱ in human dermal papilla cells. In vivo, the hair growth promoting effect was evaluated using hair growth visual scoring. BLH308 reduced hair loss in testosterone propionateinduced hair loss C57BL/6N mice by promoting the onset of growing phase of the hair growth cycle. Conclusions: Our results showed that BLH308 can promote hair regeneration and repair hair loss, and is a potential novel candidate for the treatment of androgenetic alopecia.

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